NLRP3炎症小体及其下游炎症因子在犬乳腺肿瘤组织中的表达  

Expression of NLRP3 Inflammasome Signal Associated Genes in Canine Mammary Tumors and Clinical Significance

作　　者：李妍 蓝婷英 庞博 杨晓农[1,2] 黄坚 LI Yan;LAN Tingying;PANG Bo;YANG Xiaonong;HUANG Jian(College of Animal Husbandry and Veterinary Medicine, Southwest Minzu University, Chengdu 610041, China;Veterinary Teaching Hospital, Southwest Minzu University, Chengdu 610041, China)

机构地区：[1]西南民族大学畜牧兽医学院,成都610041 [2]西南民族大学教学动物医院,成都610041

出　　处：《畜牧兽医学报》2022年第4期1252-1258,共7页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：西南民族大学中央高校基本科研业务费专项(2019NQN41);四川省科技厅面上项目(2019YFN0133)。

摘　　要：本研究旨在明确NLRP3炎症小体及下游炎症因子在犬乳腺肿瘤中的表达及临床意义。采用RT-qPCR方法检测45例犬乳腺肿瘤组织(15例良性肿瘤和30例恶性肿瘤)以及16例肿瘤旁正常乳腺组织中的NLRP3、Caspase-1、IL^(-1)β和IL^(-1)8 mRNA的表达水平,并分析NLRP3与肿瘤临床病理学特征的关系(肿瘤大小、肿瘤类型、组织恶性分级和预后)。结果显示,与肿瘤旁正常乳腺组相比,NLRP3炎症小体及下游炎症因子mRNA在肿瘤组织中均存在不同程度的表达上调,且恶性肿瘤的NLRP3(P=0.006)和Caspase-1(P=0.048)相对表达量均显著高于良性肿瘤。在恶性肿瘤中,NLRP3炎症小体的转录表达在肿瘤类型(P=0.029)和恶性等级(P=0.049)中存在显著差异。本研究提示,NLRP3炎症小体的表达上调可能导致犬乳腺组织发生肿瘤化和肿瘤恶变,是疾病诊断和恶性特征评估的重要指标之一。The aim of this study was to investigate the expression and clinical significance of NLRP3 inflammasome and downstream inflammatory genes in canine mammary tumors(CMTs).The mRNA expression levels of NLRP3,Caspase-1,IL^(-1) and IL^(-1)8 in 45 CMTs(15 benign tumors and 30 malignant tumors)and 16 tumor adjacent normal mammary gland tissues were detected by real-time quantitative polymer chain reaction.The relationship between NLRP3 and clinicopathological features(tumor size,tumor subtype,histological malignant grade and prognosis)was also analyzed.The results showed that the mRNA expression of NLRP3 and downstream inflammatory factors were up-regulated in tumor tissues compared with normal mammary tissues,and the relative mRNA expression of NLRP3(P=0.006)and Caspase-1(P=0.048)in MCMTs was significantly higher than that in BCMTs.Besides,the expression of NLRP3 mRNA in MCMTs was statistically different in tumor type(P=0.029)and malignant grade(P=0.049).This study suggests that up-regulation of NLRP3 expression may promote canine mammary tumorigenesis,and could be a diagnostic predictor for CMTs and its malignant features.

关 键 词：乳腺肿瘤 NLRP3 基因表达 犬 

分 类 号：S857.4[农业科学—临床兽医学] S857.26[农业科学—兽医学]