赛拉嗪-咪达唑仑复合麻醉剂对山羊大脑和海马NO/cGMP信号转导系统及神经递质的影响  

Effects of Compound Anesthetics of Xylazine and Midazolam on NO/cGMP Signal Transduction System and Neurotransmitters in the Brain and Hippocampus of Goats

作　　者：张志恒 白荟 彭立颖[1] 张思学[1] 贾冰[1] 魏成威[1] 高利[1] ZHANG Zhiheng;BAI Hui;PENG Liying;ZHANG Sixue;JIA Bing;WEI Chengwei;GAO Li(College of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, China)

机构地区：[1]东北农业大学动物医学学院,哈尔滨150030

出　　处：《畜牧兽医学报》2022年第4期1289-1297,共9页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：国家自然科学基金(31572580,31372491);国家重点研发计划项目(2017YFD0501601-2)。

摘　　要：旨在研究赛拉嗪复合咪达唑仑对山羊的麻醉效果,并检测中枢神经递质及NO/cGMP信号转导系统的变化,为山羊麻醉提供新的方法,并明确该复合制剂的全身麻醉作用机理。山羊肌内注射复合麻醉剂1.31 mL·kg^(-1),对照组注射生理盐水,观察动物的行为学变化,监测山羊的生理指标并评估麻醉效果,分别于麻醉诱导期、麻醉期、恢复Ⅰ期、恢复Ⅱ期采用高效液相色谱法检测大脑及海马组织中神经递质的含量以及NO/cGMP通路的变化。结果显示:1)赛拉嗪-咪达唑仑复合制剂能快速诱导山羊麻醉,诱导时间(5.63±2.35)min,麻醉维持时间(56.72±5.38)min,具有良好的镇静、镇痛及肌松作用,对山羊的基础生理参数影响小;2)复合制剂麻醉期间抑制大脑及海马组织中去甲肾上腺素(norepinephrine,NE)、多巴胺(dopamine,DA)、天门冬氨酸(aspartic acid,Asp)和谷氨酸(glutamic acid,Glu)等神经递质的表达,增加5-羟色胺(serotonin,5-HT)、5-羟吲哚乙酸(5-hydroxyindole acetic acid,5-HIAA)、海马中甘氨酸(glycine,Gly)及大脑中γ-氨基丁酸(γ-aminobutyric acid,GABA)的含量;3)复合制剂麻醉期间抑制大脑及海马组织中NO、NOS及cGMP的表达。赛拉嗪-咪达唑仑复合制剂对山羊麻醉效果较好,通过影响大脑与海马中NO/cGMP信号转导系统和多种神经递质的表达,发挥中枢麻醉机制。The goals of this study were to study the anesthetic effect of xylazine combined with midazolam on goats,and to detect the effects of combined anesthesia on central neurotransmitters and NO/cGMP signal transduction system,so as to provide a new method for goat anesthesia and to clarify the mechanism of general anesthesia of combined anesthesia.Goats received 1.31 mL·kg^(-1) combined anesthetics with intramuscular injection,and the control group received normal saline.The behavioral changes of the animals were observed,and the physiological indexes and anesthesia effects were monitored.The contents of neurotransmitters and NO/cGMP pathway in the brain and hippocampus were detected by HPLC during induction,anesthesia,recovery I and recoveryⅡ,respectively.Results were as follows:1)xylazine-midazolam compound could induce anesthesia rapidly in goats,and the induction time was(5.63±2.35)min.Anesthesia maintenance time(56.72±5.38)min.The compound exhibited well sedation,analgesia and muscle relaxation effects,and has little effect on basic physiological parameters of goats;2)During anesthesia,compound drugs inhibit the expression of norepinephrine(NE),dopamine(DA),aspartic acid(Asp)and glutamic acid(Glu)both in the brain and hippocampus,and increase the contents of serotonin(5-HT),5-hydroxyindole acetic acid(5-HIAA),glycine(Gly)in hippocampus andγ-aminobutyric acid(GABA)in the brain;3)During the anesthetic period,the compound inhibited the expression of NO,NOS and cGMP in the brain and hippocampus.We can conclude that the xylazine-midazolam compound has a good anesthetic effect on goats,and exerted the central anesthesia mechanism by affecting the NO/cGMP signal transduction system and the expression of various neurotransmitters in the brain and hippocampus.

关 键 词：山羊 赛拉嗪 咪达唑仑 NO/CGMP GABA 神经递质 

分 类 号：S859.791[农业科学—临床兽医学]