Cell-matrix reciprocity in 3D culture models with nonlinear elasticity  

作　　者：Kaizheng Liu Maury Wiendels Hongbo Yuan Changshun Ruan Paul H.J.Kouwer 

机构地区：[1]Research Center for Human Tissue and Organs Degeneration,Institute of Biomedicine and Biotechnology,Shenzhen Institutes of Advanced Technology,Chinese Academy of Sciences,Shenzhen,518055,PR China [2]Radboud University,Institute for Molecules and Materials,Heyendaalseweg 135,6525 AJ,Nijmegen,the Netherlands [3]Institute of Biophysics,Hebei University of Technology,Tianjin,300401,PR China [4]Molecular Imaging and Photonics,Chemistry Department,KU Leuven,Celestijnenlaan 200F,3001,Heverlee,Belgium

出　　处：《Bioactive Materials》2022年第3期316-331,共16页生物活性材料（英文）

基　　金：We thank Paula de Almeida for her contribution to describe the origins of strain stiffening in semiflexible networks.This project has received funding from the European Union’s Horizon 2020 research and innovation programme under the Marie Skłodowska-Curie grant agreements No 642687;and the National Natural Science Foundation of China[Grant Nos.81703584 and 51803046];the Youth Innovation Promotion Association of CAS[Grant No.2019350];the Guangdong Natural Science Foundation[Grant No.2019A1515011277];and the Shenzhen Fundamental Research Foundation[Grant No.JCYJ20180507182237428].Schematic images in the ToC graphic and Fig.1 were created in Biorender.com.

摘　　要：Three-dimensional(3D)matrix models using hydrogels are powerful tools to understand and predict cell behavior.The interactions between the cell and its matrix,however is highly complex:the matrix has a profound effect on basic cell functions but simultaneously,cells are able to actively manipulate the matrix properties.This(mechano)reciprocity between cells and the extracellular matrix(ECM)is central in regulating tissue functions and it is fundamentally important to broadly consider the biomechanical properties of the in vivo ECM when designing in vitro matrix models.This manuscript discusses two commonly used biopolymer networks,i.e.collagen and fibrin gels,and one synthetic polymer network,polyisocyanide gel(PIC),which all possess the characteristic nonlinear mechanics in the biological stress regime.We start from the structure of the materials,then address the uses,advantages,and limitations of each material,to provide a guideline for tissue engineers and biophysicists in utilizing current materials and also designing new materials for 3D cell culture purposes.

关 键 词：Artificial extracellular matrices Mechanical reciprocity COLLAGEN FIBRIN POLYISOCYANIDES 

分 类 号：R318[医药卫生—生物医学工程]