秦薯5号甘薯茎尖分生组织培养脱毒及种苗快繁技术研究  

Studies on Virus-free Meristem Culture and Rapid Propagation of Sweet Potato Qinshu 5

作　　者：秦静远[1] 朱渭兵 QIN Jingyuan;ZHU Weibing(Yangling Vocational and Technical College,Yangling,Shaanxi 712100,China;Yanging Jinshu Seed Industry Technology Co.,Ltd.Yangling,Shaanxi 712100,China)

机构地区：[1]杨凌职业技术学院,陕西杨凌712100 [2]杨凌金薯种业科技有限公司,陕西杨凌712100

出　　处：《陕西农业科学》2022年第3期8-10,91,共4页Shaanxi Journal of Agricultural Sciences

基　　金：陕西省2021年重点研发计划项目(2021NY-078)。

摘　　要：为获得秦薯5号甘薯脱毒苗用于规模化种苗生产,研究了秦薯5号茎尖分生组织培养各阶段的培养基,适宜的茎尖分生组织芽诱导培养基为MS+6-BA0.5mg/L+IAA0.2 mg/L+GA_(3)0.05 mg/L+蔗糖3%,适宜的试管苗增殖培养基为MS+6-BA0.1 mg/L+NAA0.05 mg/L+蔗糖3%+琼脂0.6%,1/2MS+NAA0.01 mg/L+蔗糖2%+琼脂0.6%适宜生根培养,繁殖倍数可达5.8。试管苗炼苗后移栽至泥炭与珍珠岩(2∶1)混合基质中,成活率可达98%以上。In order to obtain virus-free plantlets of sweet potato Qinshu 5 for large-scale seedling production,the culture medium of meristem culture of Qinshu 5 was studied,the suitable medium for meristem shoots induction was MS+6-BA0.5 mg/L+IAA 0.2 mg/L+GA_(3)0.05 mg/L+sucrose 3%,the suitable medium for in vitro plantlet multiplication was MS+6-BA 0.1 mg/L+NAA 0.05 mg/L+sucrose 3%+agar 0.6%,the suitable medium for rooting was 1/2 MS+NAA 0.01 mg/L+sucrose 2%+agar 0.6%,the multiplication multiple was 5.8.The survival rate of plantlets was over 98%when they were transplanted into the mixed media of peat and perlite(2∶1).

关 键 词：甘薯 秦薯5号 茎尖培养脱毒 快繁 

分 类 号：S531[农业科学—作物学]