粘质沙雷氏菌YW-3-10灵菌红素发酵条件优化  

Optimization of Fermentation Condition for Prodigiosin Production of Serratia Marcescen YW-3-10

作　　者：洪伟[1] 孙浩浩 薛正莲[2] HONG Wei;SUN Haohao;XUE Zhenglian(The Department of Biological Engineering,Wuhu Institute of Technology,Wuhu 241003,Chins;College of Biological and Food Engineering,Anhui Polytechnic University,Wuhu 241000,China)

机构地区：[1]芜湖职业技术学院生物工程学院,安徽芜湖241003 [2]安徽工程大学生物与食品工程学院,安徽芜湖241000

出　　处：《赣南师范大学学报》2022年第3期85-90,共6页Journal of Gannan Normal University

基　　金：安徽省高校自然科学重点项目(KJ2020A0920);安徽省高校自然科学重点项目(KJ2016A765);安徽省高校自然科学重点项目(KJ2019A0625);芜湖职业技术学院“优秀中青年骨干教师”(2018-2021)建设项目。

摘　　要：近年来灵菌红素由于其广泛的抗菌效果、免疫抑制活性及抗肿瘤活性得到广泛关注,实验室经过筛选和ARTP诱变得到一株灵菌红素产量较高的粘质沙雷氏菌菌株YW-3-10,在对YW-3-10发酵条件进行单因素实验的基础上在进行Box-Behnken中心组合试验和响应面分析,确定其摇瓶发酵产灵菌红素的最佳条件如下:甘油32 ml/L,胰蛋白胨17 g/L,甘氨酸1.5 g/L,NaCl 5 g/L,发酵温度28℃,转速200 rpm,装液量30 mL/250 mL,接种量4%,灵菌红素产量达3.285±0.0257 g/L,较初始条件灵菌红素产量有显著提升.Due to broad application in antibacterial effect ion,immunosuppressive activity and anti-tumor activity,prodigiosin get widespread attention in recent years.Through screening and ARTP our laboratory obtained a high prodigiosin yield Serratia marcescen s strain YW-3-10.Based on the single factor experiments,Box-Behnken central composite design and response surface analysis was carried out,and the optimal conditions for the production of prodigiosin of Serratia marcescen YW-3-10 was:32 ml/L lycerol,17 g/L tryptone,1.5 g/L glycine,5 g/L NaCl,fermentation temperature:28℃,rotational:200 rpm,volume of broth:30 mL/250 mL,inoculum:4%.Under this condition,the production of prodigiosin reached 3.285±0.0257 g/L,which is significantly higher than the initial conditions.

关 键 词：粘质沙雷氏菌 灵菌红素 发酵优化 响应面分析 

分 类 号：Q78[生物学—分子生物学]