絮凝菌的筛选及乳制品废水培养条件优化  

Screening of Flocculating Microorganism and its Culture Conditions Optimization Based on Dairy Wastewater as Culture Medium

作　　者：魏炜[1] 陈令薇 马振林 崔鹏 WEI Wei;CHEN Lingwei;MA Zhenlin;CUI Peng(School of Municipal and Environmental Engineering,Shenyang Jianzhu University,Shenyang,China,110168)

机构地区：[1]沈阳建筑大学市政与环境工程学院,辽宁沈阳110168

出　　处：《沈阳建筑大学学报（自然科学版）》2022年第1期186-192,共7页Journal of Shenyang Jianzhu University：Natural Science

基　　金：国家自然科学基金项目(51908377)。

摘　　要：目的研究廉价乳制品废水作为培养基筛选高效絮凝菌,并对其进行培养条件优化试验,旨在降低絮凝剂生产成本的同时筛选出更稳定的优势菌种。方法以稻田土壤、活性污泥为菌种来源,通过初筛以及复筛,共选出24株产絮菌,其中菌株C2絮凝效果最佳,并对菌株C2进行紫外诱变,得到一株高效产絮菌C2-5,并分别外加不同量葡萄糖、尿素、K_(2)HPO_(4)和KH_(2)PO_(4)以及改变pH值、发酵培养时间、发酵培养温度、发酵培养转速、接种量对产絮菌C2-5絮凝效果进行试验。结果单因素试验得出产絮菌C2-5的最佳培养条件:外加质量浓度为0.5 g/L的尿素,质量浓度为5 g/L的K_(2)HPO_(4),质量浓度为2 g/L的KH_(2)PO_(4),pH值为7,培养时间为48 h,培养温度为30℃,培养转速为150 r/min,接种量为6%。结论在最佳培养条件下,通过分离选育并诱变得到的高效产絮菌C2-5对高岭土悬浮液的絮凝效果明显。In order to reduce flocculant production cost and screen out more stable dominant strains,dairy wastewater as a cheap medium of efficient flocculant strains and the optimal culture conditions were studied.Paddy soil and activated sludge as the source of strains,24 flocculent strains were selected through preliminary screening and re-screening.Among them,strain C2 had the best flocculation effect.High efficient flocculent bacteria C2-5 was obtained by UV mutagenesis.The flocculation effect of flocculent bacteria C2-5 was tested by adding glucose,urea,K_(2)HPO_(4) and KH_(2)PO_(4),and changing pH value,fermentation time,fermentation temperature,fermentation speed and inoculation concentration.The best culture conditions for flocculent strain C2-5 through single factor experiments were urea with a mass concentration of 0.5 g/L,K_(2)HPO_(4) with a mass concentration of 5 g/L,KH_(2)PO_(4) with a mass concentration of 2 g/L,and pH 7,the culture time 48 h,the culture temperature 30℃,the culture speed 150 r/min,and the inoculum concentration 6%.Under the optimal flocculation conditions,the high-efficiency flocculant-producing bacteria C2-5 by separation,breeding and induction has obvious flocculation effect on the kaolin suspension.

关 键 词：乳制品废水 微生物絮凝剂 紫外诱变 培养条件 

分 类 号：TU391[建筑科学—结构工程]