基于高通量测序探讨参附桃红承气汤对脓毒症大鼠心肌抑制的影响  

Investigation on the effect of Shenfu Taohong Chengqi Decoction on myocardial inhibition in septic rats based on high-throughput sequencing

作　　者：潘振亚 王艳鹏 赵娜 邓旻[1] PAN Zhenya;WANG Yanpeng;ZHAO Na;DENG Min(Department of Emergency Medicine,Hangzhou Red Cross Hospital,Hangzhou310003,China)

机构地区：[1]杭州市红十字会医院急诊科,浙江杭州310003

出　　处：《中国现代医生》2022年第7期25-30,F0003,共7页China Modern Doctor

基　　金：浙江省医药卫生科技计划项目(2020KY734)。

摘　　要：目的基于高通量测序探讨参附桃红承气汤对脓毒症大鼠心肌抑制的影响。方法30只Wistar大鼠随机分为模型组、实验组、对照组,每组各10只。模型组及实验组大鼠采用腹腔注射脂多糖的方法建立脓毒症模型,实验组大鼠造模前3 d至处死前给予参附桃红承气汤灌胃,模型组和对照组给予相同量的生理盐水灌胃。造模成功后12 h观察各组大鼠一般情况及死亡率,采用超声检测大鼠心功能,采用ELISA方法检测大鼠血清CK-MB、cTNI水平,电镜下观察大鼠心肌细胞结构,采用16S rDNA测序分析肠道菌群变化。结果实验组大鼠死亡率(20%)低于模型组(30%);实验组大鼠CK-MB及cTNI低于模型组(P<0.05),但均高于对照组(P<0.05);在心脏超声结果方面,实验组大鼠LVEF及CO值高于模型组(P<0.05),均低于对照组(P<0.05);电镜下实验组见少许线粒体损伤,脊线断裂较少;模型组线粒体可见较多脊断裂、模糊,并且见空泡化;在肠道菌群丰度方面,厚壁菌门表达水平模型组<实验组<对照组(P<0.05),实验组和模型组大肠杆菌-志贺菌表达量明显高于对照组,中药干预后,实验组表达量小于模型组。结论参附桃红承气汤可以减轻脓毒症大鼠心肌损伤,改善心功能,调整肠道菌群结构。Objective To investigate the effect of Shenfu Taohong Chengqi Decoction on myocardial inhibition in septic rats based on high-throughput sequencing.Methods A total of 30 Wistar rats were randomly divided into the model group,the experimental group and the control group,with 10 rats in each group.The sepsis models were established by intraperitoneal injection of lipopolysaccharide in rats from the model group and the experimental group.The rats in the experimental group were given Shenfu Taohong Chengqi Decoction by gavage from 3 days before modeling to termination,and the model group and the control group were given the same amount of normal saline by gavage.During 12 hours upon successful modeling,the general conditions and mortality of rats in each group were observed.The cardiac function of rats was detected by ultrasound,the levels of serum CK-MB and cTnI in rats were measured by ELISA,the structure of rat cardiomyocytes was observed under electron microscope,and the changes of intestinal flora were analyzed by 16S rDNA sequencing.Results The mortality of rats in the experimental group(20%)was lower than that in the model group(30%).CK-MB and cTnI in the experimental group were lower than those in the model group(P<0.05),but higher than those in the control group(P<0.05).In terms of cardiac ultrasound results,the values of left ventricular ejection fraction(LVEF)and cardiac output(CO)in the experimental group were higher than those in the model group(P<0.05)and lower than those in the control group(P<0.05).Under electron microscope,a little mitochondrial damage and fewer broken ridges were observed in the experimental group.In the model group,there were many broken and fuzzy ridges and vacuolization seen in mitochondria.In terms of intestinal flora abundance,the expression level of Firmicutes in the model group was successively less than that in the experimental group and the control group(P<0.05),the expression level of Escherichia coli and Shigella spp.in the experimental group and the model group was signi

关 键 词：参附桃红承气汤 脓毒症 大鼠 心肌抑制 

分 类 号：R285.5[医药卫生—中药学]