不同甘蓝类蔬菜材料对根肿病的抗性鉴定及分子标记辅助选择  

Identification of Different Brassica oleracea Vegetables Resistance to Clubroot and Molecular Marker-assisted Selection

作　　者：蒋顺顺 刘才铭 赵宇[1] 胡文惠 吴嘉炳 李勤菲[1] 任雪松[1] 宋洪元[1] 司军[1] JIANG Shunshun;LIU Caiming;ZHAO Yu;HU Wenhui;WU Jiabing;LI Qinfei;REN Xuesong;SONG Hongyuan;SI Jun(School of Horticulture and Landscape Architecture,Southwest University,Chongqing Key Laboratory of Vegetable Science,Key Laboratory of Southern Mountain Horticulture,Ministry of Education,Chongqing 400715,China)

机构地区：[1]西南大学园艺园林学院,重庆市蔬菜学重点实验室,南方山地园艺学教育部重点实验室,重庆400715

出　　处：《中国蔬菜》2022年第4期45-50,共6页China Vegetables

基　　金：国家自然科学基金项目(31801855);重庆市技术创新与应用发展专项(cstc2019.jscx-gksbX0117,cstc2019.jscx-gksbX0143);中央高校基本科研业务费专项资金资助项目(SWU118063,XDJK2019B046);重庆市自然科学基金项目(cstc2018jcyjAX0039)。

摘　　要：根肿病是十字花科蔬菜主要病害之一,严重影响十字花科蔬菜生产,选育抗病品种是防治根肿病的有效途径之一。本试验采用灌根法苗期人工接种根肿菌4号生理小种,对29份甘蓝类蔬菜材料进行根肿病抗性鉴定;并利用与抗病基因CRb连锁的SCAR标记(OA)和与抗病基因CRb^(Kato)连锁的SSR标记(B0902)对人工接种鉴定表现免疫、高抗、抗病、耐病的材料进行分子标记辅助鉴定。结果表明:人工接种鉴定筛选出8份抗病材料,其中Cr7表现免疫,绿抗9号、青莲甘蓝表现高抗,S148、Cr5、Cr6表现抗病,S146、S296表现耐病。分子标记鉴定结果显示,4份材料含有CRb基因,8份材料含有CRb^(Kato)基因,验证了这8份抗病材料的真实性;其中Cr5、绿抗9号、青莲甘蓝、S296含有CRb基因和CRb^(Kato)基因2个抗性位点,可将这4份材料直接应用到甘蓝抗根肿病育种中。Clubroot is one of the main diseases of cruciferous vegetables seriously threating its production.Breeding resistant variety is one of the effective methods to control clubroot.This experiment adopted root irrigation and artificial inoculation methods at seedling stage on physiological race of No.4 clubroot;conducted clubroot resistance identification on 29 Brassica oleracea vegetable accessions;and using molecular marker assisted identification using SCAR marker(OA)and SSR marker(B0902)linked to disease resistance gene CRb and CRb^(Kato) on materials expressing immune,high resistant,disease resistant and disease tolerant by artificial inoculation identification.The results indicated that among 8 disease-resistant accessions screened by artificial inoculation identification,‘Cr7’showed immunity,‘Lvkang No.9’and‘Qinglianganlan’showed high resistance,‘S148’‘Cr5’and‘Cr6’showed disease resistance,‘S146’and‘S296’showed disease tolerance.Results of molecular marker identification showed that 4 accessions contained CRb gene,8 accessions contained CRb^(Kato) gene,verifying the authenticity of these 8 disease-resistant accessions.Moreover,‘Cr5’‘Lvkang No.9’‘Qinglianganlan’‘S296’accessions contained 2 resistant loci.Therefore,these 4 accessions could be directly applied to breeding of clubroot resistant cabbage.

关 键 词：甘蓝类蔬菜 根肿病 抗性鉴定 分子标记 

分 类 号：S436.35[农业科学—农业昆虫与害虫防治]