Large-sized bone defect repair by combining a decalcified bone matrix framework and bone regeneration units based on photo-crosslinkable osteogenic microgels  

作　　者：Junxiang Hao Baoshuai Bai Zheng Ci Jincheng Tang Guanhuai Hu Chengxiang Dai Mengyuan Yu Meng Li Wei Zhang Yixin Zhang Wenjie Ren Yujie Hua Guangdong Zhou 

机构地区：[1]Research Institute of Plastic Surgery,Weifang Medical University,Weifang,Shandong,China [2]Department of Plastic and Reconstructive Surgery,Shanghai 9th People’s Hospital,Shanghai Jiao Tong University School of Medicine,Shanghai Key Laboratory of Tissue Engineering,Shanghai,China [3]National Tissue Engineering Center of China,Shanghai,China [4]Institute of Regenerative Medicine and Orthopedics,Institutes of Health Central Plain,Xinxiang Medical University,Xinxiang,Henan,China [5]Cellular Biomedicine Group,Inc.,Shanghai,China

出　　处：《Bioactive Materials》2022年第8期97-109,共13页生物活性材料（英文）

基　　金：financially supported by the National Key Research and Development Program of China(2017YFC1103900);the National Natural Science Foundation of China(81871502,81701843,and 81671837);the Shanghai Excellent Technical Leader(18XD1421500);the Program of Shanghai Academic/Technology Research Leader(19XD1431100);the Shanghai Collaborative Innovation Program on Regenerative Medicine and Stem Cell Research(2019CXJQ01);the Clinical Research Plan of SHDC(No.SHDC2020CR2045B).

摘　　要：Physiological repair of large-sized bone defects is great challenging in clinic due to a lack of ideal grafts suitable for bone regeneration.Decalcified bone matrix(DBM)is considered as an ideal bone regeneration scaffold,but low cell seeding efficiency and a poor osteoinductive microenvironment greatly restrict its application in large-sized bone regeneration.To address these problems,we proposed a novel strategy of bone regeneration units(BRUs)based on microgels produced by photo-crosslinkable and microfluidic techniques,containing both the osteogenic ingredient DBM and vascular endothelial growth factor(VEGF)for accurate biomimic of an osteoinductive microenvironment.The physicochemical properties of microgels could be precisely controlled and the microgels effectively promoted adhesion,proliferation,and osteogenic differentiation of bone marrow mesenchymal stem cells(BMSCs)in vitro.BRUs were successfully constructed by seeding BMSCs onto microgels,which achieved reliable bone regeneration in vivo.Finally,by integrating the advantages of BRUs in bone regeneration and the advantages of DBM scaffolds in 3D morphology and mechanical strength,a BRU-loaded DBM framework successfully regenerated bone tissue with the desired 3D morphology and effectively repaired a large-sized bone defect of rabbit tibia.The current study developed an ideal bone biomimetic microcarrier and provided a novel strategy for bone regeneration and large-sized bone defect repair.

关 键 词：Microgels Large-sized bone defect repair Bone regeneration units PHOTO-CROSSLINKING Decalcified bone matrix 

分 类 号：R68[医药卫生—骨科学]