沙眼衣原体小鼠肺炎株呼吸道感染诱导巨噬细胞浸润并向M1极化  

Chlamydia muridarum respiratory infection induces macrophage infiltration and polarization toward M1 phenotype

作　　者：许悦悦 钮文豪 景晔 查晓宇 曾佳佳 杨帅妮 曲同兴 张虹 白虹 Xu Yueyue;Niu Wenhao;Jing Ye;Zha Xiaoyu;Zeng Jiajia;Yang Shuaini;Qu Tongxing;Zhang Hong;Bai Hong(Tianjin Key Laboratory of Cellular and Molecular Immunology,Key Laboratory of Immune Microenvironment and Diseases of Educational Ministry of China,Department of Immunology,School of Basic Medical Sciences,Tianjin Medical University,Tianjin 300070,China;Laboratory Pathology,Tianjin Rehabilitation and Recuperation Center,Tianjin 300381,China)

机构地区：[1]天津医科大学基础医学院免疫学系,国家教育部免疫微环境与疾病重点实验室,天津市细胞和分子免疫学重点实验室,天津300070 [2]天津康复疗养中心检验病理科,天津300381

出　　处：《中华微生物学和免疫学杂志》2022年第3期194-201,共8页Chinese Journal of Microbiology and Immunology

基　　金：国家自然科学基金(31870889);天津市自然科学基金(19JCZDJC35800)。

摘　　要：目的探讨沙眼衣原体小鼠肺炎株(Chlamydia muridarum,Cm)呼吸道感染对巨噬细胞浸润及向M1/M2极化的影响。方法经鼻腔给予C57BL/6小鼠1×103包涵体形成单位(inclusion-forming units,IFU)Cm建立小鼠沙眼衣原体呼吸道感染模型。流式细胞术检测肺组织中F4/80+总巨噬细胞及CD86、主要组织相容性复合体Ⅱ(major histocompatibility complexⅡ,MHCⅡ)、诱导型一氧化氮合酶(inducible nitric oxide synthase,iNOS)、CD206表达情况;实时荧光定量聚合酶链反应检测肺组织中iNOS、CD206、CCL2 mRNA表达。结果Cm呼吸道感染诱导小鼠肺组织中巨噬细胞增加,与未感染组比较,感染后第3天F4/80+巨噬细胞显著增加,并且持续到第7天;Cm感染后,M1型巨噬细胞表面标志CD86、MHCⅡ及M1型巨噬细胞相关趋化因子CCL2表达增加,巨噬细胞向M1型极化;M2型巨噬细胞表面标志CD206逐渐降低。进一步研究发现,M1型巨噬细胞活化指标iNOS在感染后增加,第7天达到高峰。结论Cm呼吸道感染后可诱导巨噬细胞在肺组织大量浸润,且向M1型巨噬细胞极化。Objective To investigate the infiltration and polarization of macrophages in mice during Chlamydia muridarum(Cm)respiratory infection.Methods C57BL/6 mice were intranasally infected with 1×103 inclusion-forming units(IFU)of Cm to establish the mouse model of Cm respiratory tract infection.The percentages of CD45+F4/80+cells and the macrophages expressing CD86,major histocompatibility complexⅡ(MHC),inducible nitric oxide synthase(iNOS)and CD206 were detected by flow cytometry.Expression of iNOS,CD206 and CCL2 at mRNA level was detected by real-time quantitative PCR.Results Cm respiratory tract infection induced the increase of macrophages in mouse lung tissues.Compared with uninfected group,CD45+F4/80+macrophages were increased significantly from day 3 and reached the peak on day 7 after Cm infection.Moreover,the expression of CD86,MHCⅡand CCL2 was increased,and the macrophages were polarized to M1 phenotype.However,the expression of M2 macrophage marker CD206 was decreased gradually.Further studies showed that iNOS expression,the indicator of M1 macrophage activation,was increased after Cm infection and reached to the top on day 7.Conclusions Cm respiratory infection could induce the infiltration of macrophages in lung tissues and promote the polarization of macrophages to M1 phenotype.

关 键 词：沙眼衣原体小鼠肺炎株 巨噬细胞 极化 炎症反应 

分 类 号：R392[医药卫生—免疫学]