Synergistic stimulation of surface topography and biphasic electric current promotes muscle regeneration  

作　　者：Indong Jun Na Li Jaehee Shin Jaeho Park Young Jun Kim Hojeong Jeon Hyuk Choi Jae-Gu Cho Byoung Chan Choi Hyung-Seop Han Jae-Jun Song 

机构地区：[1]Environmental Safety Group,Korea Institute of Science&Technology Europe(KIST-EUROPE),Saarbrücken,66123,Germany [2]Department of Otorhinolaryngology-Head and Neck Surgery,Korea University College of Medicine,Seoul,02841,Republic of Korea [3]Department of Medical Sciences,Graduate School of Medicine,Korea University,Seoul,02841,Republic of Korea [4]Center for Biomaterials,Biomedical Research Institute,Korea Institute of Science&Technology(KIST),Seoul,02792,Republic of Korea [5]Laser Surface Texturing Group,AYECLUS,Gyeonggi-do,14255,Republic of Korea

出　　处：《Bioactive Materials》2022年第5期118-129,共12页生物活性材料（英文）

基　　金：supported by a Korean National Research Foundation(NRF)grant funded by the Korea government(MSIT)(No.2017M3A9G1027929);supported by both the National Research Council of Science&Technology 424(NST)grant by the Korean government(MSIP)(CAP-17-01-KIST Europe);KIST project(2E31121).

摘　　要：Developing a universal culture platform that manipulates cell fate is one of the most important tasks in the investigation of the role of the cellular microenvironment.This study focuses on the application of topographical and electrical field stimuli to human myogenic precursor cell(hMPC)cultures to assess the influences of the adherent direction,proliferation,and differentiation,and induce preconditioning-induced therapeutic benefits.First,a topographical surface of commercially available culture dishes was achieved by femtosecond laser texturing.The detachable biphasic electrical current system was then applied to the hMPCs cultured on laser-textured culture dishes.Laser-textured topographies were remarkably effective in inducing the assembly of hMPC myotubes by enhancing the orientation of adherent hMPCs compared with flat surfaces.Furthermore,electrical field stimulation through laser-textured topographies was found to promote the expression of myogenic regulatory factors compared with nonstimulated cells.As such,we successfully demonstrated that the combined stimulation of topographical and electrical cues could effectively enhance the myogenic maturation of hMPCs in a surface spatial and electrical field-dependent manner,thus providing the basis for therapeutic strategies.

关 键 词：TOPOGRAPHY Electrical field stimulation Femtosecond laser Human myogenic precursor cell Cell therapy 

分 类 号：R318[医药卫生—生物医学工程] R337[医药卫生—基础医学]