The diameter factor of aligned membranes facilitates wound healing by promoting epithelialization in an immune way  

作　　者：Chenbing Wang Chenyu Chu Xiwen Zhao Yang Yang Chen Hu Li Liu Jidong Li Yili Qu Yi Man 

机构地区：[1]Department of Oral Implantology&National Clinical Research Center for Oral Diseases&State Key Laboratory of Oral Diseases,West China Hospital of Stomatology,Sichuan University,Chengdu,China [2]Research Center for Nano-Biomaterials,Analytical and Testing Center,Sichuan University,Chengdu 610064,China [3]State Key Laboratory of Biotherapy,West China Hospital,Sichuan University,Collaborative Innovation Center for Biotherapy,Chengdu,Sichuan 610041,China

出　　处：《Bioactive Materials》2022年第5期206-217,共12页生物活性材料（英文）

基　　金：supported by Key R&D Projects of Sichuan Science and Technology Plan(2021YFS0030);Interdisciplinary Innovation Project,West China Hospital of Stomatology Sichuan University(RD-03-202006);Research and Develop Program,West China Hospital of Stomatology Sichuan University(No.LCYJ2019-19).

摘　　要：Topographical properties,such as pattern and diameter,of biomaterials play important roles in influencing cell activities and manipulating the related immune response during wound healing.We prepared aligned electrospinning membranes with different fiber diameters,including 319±100 nm(A300),588±132 nm(A600),and 1048±130 nm(A1000),by adjusting the distance from the tip to the collector,the injection rate,and the concentration of the solution.The A300 membranes significantly improved cell proliferation and spreading and facilitated wound healing(epithelization and vascularization)with the regeneration of immature hair follicles compared to the other membranes.Transcriptomics revealed the underlying molecular mechanism that A300 could promote immune-related processes towards a pro-healing direction,significantly promoting keratinocyte migration and skin wound healing.All the results indicated that wound healing requires the active participation of the immune process,and that A300 was a potential candidate for guided skin regeneration applications.

关 键 词：Aligned membranes DIAMETER Wound healing EPITHELIALIZATION MMP12 

分 类 号：R318[医药卫生—生物医学工程] R45[医药卫生—基础医学]