Effect of minor amounts of β-calcium pyrophosphate and hydroxyapatite on the physico-chemical properties and osteoclastic resorption of β-tricalcium phosphate cylinders  

作　　者：B.Le Gars Santoni L.Niggli S.Dolder O.Loeffel G.A.Sblendorio R.Heuberger 

机构地区：[1]RMS Foundation,Bioceramics and Biocompatibility Group,Bischmattstrasse 12,CH-2544,Bettlach,Switzerland [2]University of Bern,Graduate School for Cellular and Biomedical Sciences,Mittelstrasse 43,CH-3012,Bern,Switzerland [3]University of Bern,Department for BioMedical Research(DBMR),Murtenstrasse 35,CH-3008,Bern,Switzerland [4]RMS Foundation,Materials Group,Bischmattstrasse 12,CH-2544,Bettlach,Switzerland [5]EPFL,Ecole Polytechnique Federale de Lausanne,Construction Materials Laboratory,Station 12,CH-1015,Lausanne,Switzerland

出　　处：《Bioactive Materials》2022年第4期222-235,共14页生物活性材料（英文）

基　　金：the Swiss National Science Foundation for its funding(Grant no.200021_169027).

摘　　要：β-Tricalcium Phosphate(β-TCP),one of the most used bone graft substitutes,may contain up to 5 wt%foreign phase according to standards.Typical foreign phases includeβ-calcium pyrophosphate(β-CPP)and hydroxyapatite(HA).Currently,the effect of small amounts of impurities on β-TCP resorption is unknown.This is surprising since pyrophosphate is a very potent osteoclast inhibitor.The main aim of this study was to assess the effect of small β-CPP fractions(<1 wt%)on the in vitro osteoclastic resorption of β-TCP.A minor aim was to examine the effect of β-CPP and HA impurities on the physico-chemical properties of β-TCP powders and sintered cylinders.Twenty-six batches of β-TCP powder were produced with a Ca/P molar ratio varying between 1.440 and 1.550.Fifteen were further processed to obtain dense and polished β-TCP cylinders.Finally,six of them,with a Ca/P molar ratio varying between 1.496(1 wt% β-CPP)and 1.502(1 wt% HA),were incubated in the presence of osteoclasts.Resorption was quantified by white-light interferometry.Osteoclastic resorption was significantly inhibited by β-CPP fraction in a linear manner.The presence of 1% β-CPP reduced β-TCP resorption by 40%,which underlines the importance of controllingβ-CPP content when assessing β-TCP biological performance.

关 键 词：BIOCERAMICS Calcium phosphate β-Tricalcium phosphate β-Calcium pyrophosphate HYDROXYAPATITE Osteoclastic resorption 

分 类 号：R318[医药卫生—生物医学工程] R68[医药卫生—基础医学]