A functional PVA aerogel-based membrane obtaining sutureability through modified electrospinning technology and achieving promising anti-adhesion effect after cardiac surgery  

作　　者：Dawei Jin Shuofei Yang Shuting Wu Meng Yin Haizhu Kuang 

机构地区：[1]Department of Cardiothoracic Surgery,Shanghai Children’s Medical Center,School of Medicine,Shanghai Jiao Tong University,1678 Dong Fang Road,Shanghai,200127,People’s Republic of China [2]Department of Vascular Surgery,Renji Hospital,School of Medicine,Shanghai Jiao Tong University,160 Pujian Road,Shanghai,200127,People’s Republic of China [3]Department of Pharmacy,The Third Affiliated Hospital(The Affiliated Luohu Hospital)of Shenzhen University,Shenzhen,518001,Guangdong Province,People’s Republic of China

出　　处：《Bioactive Materials》2022年第4期355-366,共12页生物活性材料（英文）

基　　金：The National Natural Science Fund of China(81873923);Shanghai Science and Technology Development Fund(20Y11910600,18441901900)supported this study.

摘　　要：Pericardial barrier destruction,inflammatory cell infiltration,and fibrous tissue hyperplasia,trigger adhesions after cardiac surgery.There are few anti-adhesion materials that are both functional and sutureable for pericardial reconstruction.Besides,a few studies have reported on the mechanism of preventing pericardial adhesion.Herein,a functional barrier membrane with sutureability was developed via a modified electrospinning method.It was composed of poly(L-lactide-co-caprolactone)(PLCL)nanofibers,poly(vinyl alcohol)(PVA)aerogel,and melatonin,named PPMT.The PPMT had a special microstructure manifested as a staggered arrangement of nanofibers on the surface and a layered macroporous aerogel structure in a cross-section.Besides providing the porosity and hydrophilicity obtained from PVA,the structure also had suitable mechanical properties for stitching due to the addition of PLCL nanofibers.Furthermore,it inhibited the proliferation of fibroblasts by suppressing the activation of Fas and P53,and achieved anti-inflammatory effects by affecting the activity of inflammatory cells and reducing the release of pro-inflammatory factors,such as interleukin 8(IL-8)and tumor necrosis factorα(TNF-α).Finally,in vivo transplantation showed that it up-regulated the expression of matrix metalloproteinase-1(MMP1)and tissue inhibitor of metalloproteinase-1(TIMP1),and down-regulated the expression of Vinculin and transforming growth factorβ(TGF-β)in the myocardium,thereby reducing the formation of adhesions.Collectively,these results demonstrate a great potential of PPMT membrane for practical application to anti-adhesion.

关 键 词：ANTI-ADHESION PVA aerogel-Based membrane Sutureability Modified electrospinning technology Pericardial reconstruction 

分 类 号：R318[医药卫生—生物医学工程]