Profiling the responsiveness of focal adhesions of human cardiomyocytes to extracellular dynamic nano-topography  

作　　者：Huaiyu Shi Xiangjun Wu Shiyang Sun Chenyan Wang Zacharias Vangelatos Ariel Ash-Shakoor Costas P.Grigoropoulos Patrick T.Mather James H.Henderson Zhen Ma 

机构地区：[1]Department of Biomedical&Chemical Engineering,Syracuse University,Syracuse,NY,13244,USA [2]BioInspired Syracuse Institute for Materials and Living Systems,Syracuse University,Syracuse,NY,13244,USA [3]Department of Mechanical Engineering,University of California,Berkeley,PA,94720,USA [4]Department of Chemical Engineering,Bucknell University,Lewisburg,PA,17837,USA

出　　处：《Bioactive Materials》2022年第4期367-377,共11页生物活性材料（英文）

基　　金：supported by the NIH NICHD[R01HD101130];NSF[CBET-1804875,CBET-1943798 and CMMI-2130192];NSF[DMR-1609523 and CMMI-2022421];Syracuse University intramural CUSE Grant;Gerber Grant;BioInspired Institute Seed Grant.

摘　　要：Focal adhesion complexes function as the mediators of cell-extracellular matrix interactions to sense and transmit the extracellular signals.Previous studies have demonstrated that cardiomyocyte focal adhesions can be modulated by surface topographic features.However,the response of focal adhesions to dynamic surface topographic changes remains underexplored.To study this dynamic responsiveness of focal adhesions,we utilized a shape memory polymer-based substrate that can produce a flat-to-wrinkle surface transition triggered by an increase of temperature.Using this dynamic culture system,we analyzed three proteins(paxillin,vinculin and zyxin)from different layers of the focal adhesion complex in response to dynamic extracellular topographic change.Hence,we quantified the dynamic profile of cardiomyocyte focal adhesion in a time-dependent manner,which provides new understanding of dynamic cardiac mechanobiology.

关 键 词：Stimuli-responsive biomaterials Shape memory polymer Focal adhesion Costamere Dynamic mechanobiology Human induced pluripotent stem cells 

分 类 号：R542.2[医药卫生—心血管疾病] R318[医药卫生—内科学]