益生菌对早产巴马猪生后肺发育的影响  

Impact of probiotics on the lung development of Bama minipig after premature birth

作　　者：谢亮[1] 杨森[1] 周丹[1] 陈婷婷 张莹 卢婧[3] 王秋 石芳[4] 刘洋 伏洪玲 刘瀚旻[1] Xie Liang;Yang Sen;Zhou Dan;Chen Tingting;Zhang Ying;Lu Jing;Wang Qiu;Shi Fang;Liu Yang;Fu Hongling;Liu Hanmin(Department of Pediatric Pulmonology and Immunology,West China Second University Hospital,Sichuan University,Chengdu 610041,China;Key Laboratory of Birth Defects and Related Diseases of Women and Children(Sichuan University),Ministry of Education,West China Second University Hospital,Sichuan University,Chengdu 610041,China;the Joint Laboratory for Lung Development and Related Diseases of West China Second University Hospital,Sichuan University and School of Life Sciences of Fudan University,Chengdu 610041,China;National Health Commission Key Laboratory of Chronobiology(Sichuan University),West China Second University Hospital,Sichuan University,Chengdu 610041,China)

机构地区：[1]四川大学华西第二医院小儿呼吸免疫科,成都610041 [2]四川大学华西第二医院出生缺陷与相关妇儿疾病教育部重点实验室(四川大学),成都610041 [3]四川大学华西第二医院/复旦大学生命科学学院肺发育与相关疾病联合实验室,成都610041 [4]四川大学华西第二医院国家卫生健康委员会时间生物学重点实验室,成都610041

出　　处：《中华儿科杂志》2022年第4期297-301,共5页Chinese Journal of Pediatrics

基　　金：国家自然科学基金区域创新发展联合基金(U21A20333);四川省中央引导地方科技发展专项(2021ZYD0105)。

摘　　要：目的探索益生菌干预在早产巴马猪生后肺脏发育中的作用。方法动物实验研究,2020年4月至2021年10月利用已成功建立的30只早产巴马猪模型,将仔猪分为早产组(妊娠104 d出生)、足月组(妊娠113 d出生)、早产益生菌干预组(妊娠104 d出生后第3天开始给予口服益生菌)和足月益生菌干预组(妊娠113 d出生后第3天开始给予口服益生菌),分别记录早产组和足月组仔猪出生后即刻、生后4、9、21 d以及早产益生菌干预组和足月益生菌干预组仔猪生后9 d后的体重并收集肺组织、回肠组织和肠道内容物。通过比较体重、辐射状肺泡计数来评估仔猪发育情况,通过测量回肠绒毛高度评估回肠发育情况,通过菌群16 S核糖体RNA分析评估回肠内容物的菌类构成,组间比较采用t检验。结果30只仔猪中雌性16只、雄性14只。早产组和足月组各12只,早产益生菌干预组和足月益生菌干预组各3只。早产组仔猪生后4、9、21 d的体重均分别低于足月组生后4、9、21 d[(507±27)比(694±56)g、(620±35)比(1092±154)g、(1660±210)比(2960±418)g,t=2.96、2.99、2.78,均P<0.05],早产组仔猪出生9 d的辐射状肺泡计数低于足月组9 d的仔猪[(4.00±0.29)比(6.11±0.35)个,t=4.64,P<0.01]。早产组和足月组仔猪在生后不同时间点有不同的高丰度回肠菌属,生后4 d时分别为大肠杆菌志贺菌属26.63%和肠球菌属30.48%;出生9 d时分别为苏黎世杆菌属35.94%和乳杆菌属27.33%;出生21 d时分别为大肠杆菌志贺菌属28.02%和乳杆菌属46.29%。早产组9 d仔猪的回肠绒毛高度小于足月组9 d的仔猪[(297±21)比(411±32)μm,t=3.01,P=0.007]。早产益生菌干预组和足月益生菌干预组的仔猪体重、辐射状肺泡计数差异均无统计学意义[(692±36)比(767±67)g、(5.44±0.34)比(5.89±0.26)个,t=0.74、1.04,均P>0.05]。早产益生菌干预组及足月益生菌干预组的回肠优势菌属均为苏黎世杆菌属,早产益生菌干预�Objectives To examine the impact of probiotics on the lung development of preterm birth of Bama pig.Methods From April 2020 to October 2021,this animal experimental research was performed by setting up preterm(birth at gestation 104 d),full-term(birth at gestation 113 d),preterm with probiotics(birth at gestation 104 d treated with probiotics given at 3 d after birth),and full-term with probiotics(birth at gestation 113 d treated with probiotics given at 3 d after birth)groups and using the preterm Bama minipig model,the body weights were recorded and lung,ileum,and intestinal content samples were collected at birth,4 days,9 days,and 21 days after births of the piglets in preterm and full-term groups,the same samples were collected on 9 days after births of the piglets in preterm with probiotics and full-term with probiotics groups.The body weight and radial alveolar counts(RAC)were compared to evaluate the lung development of the piglets.The lengths of ileal villus were compared to evaluate the development of ileum.The composition structures of bacteria in ileum were analyzed by 16 S rRNA sequencing.The statistical analyses between different groups were performed by t test.Results There were totally 30 piglets(16 female piglets and 14 male piglets)involving 12 piglets in preterm and full-term groups respectively and 3 piglets in preterm with probiotics and full-term with probiotics groups respectively.The body weights of the piglets in preterm group were lower than those in full-term group at 4,9 and 21 d after birth((507±27)vs.(694±56)g,(620±35)vs.(1092±154)g,(1660±210)vs.(2960±418)g,t=2.96,2.99,2.78,all P<0.05).The alveolarization of the preterm piglets at 9 days after birth was significantly lower than that of the full-term piglets at the equivalent time point(4.00±0.29 vs.6.11±0.35,t=4.64,P<0.01).The bacteria genus with the highest abundance in ileum were all different between the preterm and the full-term groups at 4,9 and 21 d after birth(4 d Escherichia-Shigella(26.63%)and Enterococcus(30.48%)resp

关 键 词：早产 猪 肺 有益菌种 

分 类 号：R722.6[医药卫生—儿科]