在体记录清醒小鼠丘脑网状核场电位多通道电生理技术平台的建立和验证  

Establishment and validation of a multichannel electrophysiological platform for recording thalamic reticular nucleus potential in vivo of freely moving mice

作　　者：姚云霞 张树卓 苏瑞斌 李明媛 YAO Yun-xia;ZHANG Shu-zhuo;SU Rui-bin;LI Ming-yuan(Tianjin Enterprise Key Laboratory for Application Research of Hyaluronic Acid,College of Biotech-nology,Tianjin University of Science&Technology,Tianjin 300457,China;Beijing Key Laboratory of Neuropsychopharmacology,State Key Laboratory of Toxicology and Medical Countermeasures,Institute of Pharmacology and Toxicology,Academy of Military Medical Sciences,Beijing 100850,China)

机构地区：[1]天津科技大学生物工程学院,天津市透明质酸应用研究企业重点实验室,天津300457 [2]军事科学院军事医学研究院毒物药物研究所,抗毒药物与毒理学国家重点实验室,神经精神药理学北京市重点实验室,北京100850

出　　处：《中国药理学与毒理学杂志》2022年第3期197-205,共9页Chinese Journal of Pharmacology and Toxicology

基　　金：天津市教委“十三五综投”一般科研项目(2018KJ124);天津市透明质酸应用研究企业重点实验室开放基金(KTRDHAY201906)。

摘　　要：目的 建立能够在体记录清醒自由活动小鼠丘脑网状核场电位多通道电生理记录平台。方法 在小鼠丘脑网状核植入记录电极,采用OmniPlex神经数据采集系统记录清醒自由活动小鼠丘脑网状核的局部场电位,用Offline Sorter和NeuroExplore等软件对局部场电位滤波,对滤波后的γ和θ振荡场电位进行频谱分析。小鼠ip给予γ-氨基丁酸A型受体拮抗剂荷包牡丹碱2.25 mg·kg^(-1)验证记录到的γ振荡;随后观察ip给予T型钙通道拮抗剂TTA-A2 1.0 mg·kg^(-1)后清醒自由活动小鼠丘脑网状核γ和θ振荡;最后用尼氏染色验证电极植入位点。结果 在清醒自由活动小鼠丘脑网状核记录到γ振荡局部场电位。与基线相比,荷包牡丹碱降低清醒自由活动小鼠丘脑网状核30~80 Hz和35~45 Hz γ振荡功率谱(P<0.05,P<0.01),而对θ振荡无影响。与基线相比,TTA-A2降低清醒自由活动小鼠丘脑网状核γ和θ振荡功率谱(P<0.01)。尼氏染色结果显示,丘脑网状核处可见清晰电极轨迹,电极尖端处于目标脑区,表明电极植入位点正确。结论 建立了清醒自由活动小鼠丘脑网状核场电位记录平台,为探究神经网络功能及新药研发奠定实验基础。OBJECTIVE To establish a method for recording local field potential oscillation in the thalamic reticular nucleus(TRN) of freely moving mice. METHODS Recording electrodes were implanted in the TRN of mice. The Omni Plex neural data acquisition system was used to record the local field potential of the TRN in freely moving mice. Offline Sorter and NeuroExplore or other software was used to analyze the γ and θ oscillations by filtering the field potential. The γ oscillation recorded was verified during the application of bicuculline(2.25 mg·kg^(-1), ip), a γ-aminobutyric acid type A receptor antagonist, and the activity patterns were further analyzed with(R)-2-(4-cyclopropylphenyl)-N-(1-(5-(2, 2, 2-trifluoroethoxy) pyridin-2-yl) ethyl) acetamide(TTA-A2)(1.0 mg · kg^(-1), ip), a T-type calcium channel antagonist. The site of electrode implantation was verified by Nissner staining. RESULTS Compared with baseline, bicuculline reduced the power spectrum of γ oscillation at 30-80 Hz and 35-45 Hz, respectively(P<0.05, P<0.01), but had no effect on θ oscillation, suggesting that γ oscillation was recorded in the TRN of freely moving mice. Compared with baseline, TTA-A2 reduced the θ and γ oscillation power spectrum in the TRN of freely moving mice(P<0.01). The results of Nissner staining showed that the electrode track was clear in the TRN, and the electrode tip was in the target brain region, which suggested that the location of recording electrodes was correct. CONCLUSION A multichannel electrophysiological recording platform and method are integrated for in vivo recording of field potential in the TRN of freely moving mice. This method can contribute to new drug research and studies on brain network functions.

关 键 词：多通道电生理记录平台 丘脑网状核 场电位 γ振荡 θ振荡 T型钙通道 

分 类 号：R965.2[医药卫生—药理学]