ILF2及NF90在口腔鳞状细胞癌中的表达及临床意义  

Expression and clinical significance of ILF2 and NF90 in oral squamous cell carcinoma

作　　者：王雪[1] 高雨蔚 宋红权[1] 张兴伟 杨帆 焦晓辉[1] WANG Xue;GAO Yuwei;SONG Hongquan;ZHANG Xingwei;YANG Fan;JIAO Xiaohui(Department of Oral and Maxillofacial Surgery,The First Affiliated Hospital of Harbin Medical University,Harbin 150001,China;Department of Stomatology,The First Affiliated Hospital of Heilongjiang University of Traditional Chinese Medicine)

机构地区：[1]哈尔滨医科大学附属第一医院口腔颌面外科,哈尔滨150001 [2]黑龙江中医药大学附属第一医院口腔科

出　　处：《实用肿瘤学杂志》2022年第2期137-143,共7页Practical Oncology Journal

基　　金：科技部十三五国家重点研发计划项目(编号:2016YFC1000504)。

摘　　要：目的探讨白细胞介素增强结合因子2(Interleukin enhancer-binding factor 2,ILF2)及核因子90(Nuclear factor 90,NF90)在口腔鳞状细胞癌(Oral squamous cell carcinoma,OSCC)中的表达及临床意义。方法使用免疫组织化学(Immunohistochemistry,IHC)及实时荧光定量检测(Quantitative real-time PCR,qRT-PCR)方法检测ILF2及NF90在OSCC和正常口腔黏膜(Normal oral mocusa,NOM)组织中的表达水平,并进一步分析二者表达情况与OSCC患者临床病理特征以及预后之间的关系;使用Oncomine及GEPIA数据库分析二者在不同组织中的表达情况。结果与NOM组织对比,ILF2及NF90在OSCC组织中高表达(P<0.05),二者在OSCC组织中的表达具有相关性(φ=0.540,P<0.001);Oncomine数据库结果显示ILF2及NF90同样在OSCC组织中高表达(P<0.05);GEPIA数据库结果显示ILF2及NF90在OSCC中的表达呈正相关(r=0.600,P<0.001)。ILF2及NF90的表达情况与OSCC的肿瘤分化程度、TNM分期及淋巴结是否转移相关(P<0.05);生存分析显示ILF2和NF90高表达的患者总生存期较短,且ILF2及NF90因子的高表达和肿瘤分化程度是影响患者总生存期的独立危险因素(P<0.05)。结论ILF2和NF90在OSCC中高表达,且两者具有相关性,对OSCC患者的预后有不良影响,提示二者可以作为OSCC发生发展新的诊断标志物及预后评估指标。Objective The aim of this study was to investigate the expression of interleukin enhancer binding factor 2(ILF2)and nuclear factor 90(NF90)in oral squamous cell carcinoma(OSCC)and their clinical significance.Methods Immunohistochemistry(IHC)and quantitative real-time PCR(qRT-PCR)were used to detect the expression of ILF2 and NF90 in OSCC and normal oral mucosa(NOM)tissues.The relationship between their expression and clinicopathological features and prognosis of OSCC patients was further analyzed.Oncomine and GEPIA databases were used to analyze their expression in different tissues.Results Compared with NOM tissues,ILF2 and NF90 were highly expressed in OSCC tissues(P<0.05);their expression was positively correlated in OSCC tissues(φ=0.540,P<0.001);the results of Oncomine database showed that ILF2 and NF90 were also highly expressed in OSCC tissues(P<0.05);The results of GEPIA database showed that the expression of ILF2 and NF90 in OSCC was positively correlated(r=0.600,P<0.001).The expression of ILF2 and NF90 was correlated with the degree of tumor differentiation,TNM stage and lymph node metastasis of OSCC(P<0.05).Prognostic survival analysis showed that patients with high expression of ILF2 and NF90 had a short overall survival,and the high expression of ILF2 and NF90 and the degree of tumor differentiation were independent risk factors affecting the overall survival of patients.Conclusion ILF2 and NF90 are highly expressed and correlated in OSCC,and they are correlated,and have adverse effects on the prognosis of OSCC patients,suggesting that they can be used as new diagnostic markers and prognostic indicators for the occurrence and development of OSCC.

关 键 词：口腔鳞状细胞癌 白细胞素增强结合因子2 核因子90 

分 类 号：R782[医药卫生—口腔医学]