Evaluation of Cuban Bacillus thuringiensis(Berliner,1911)(Bacillales:Bacillacea)isolates with larvicidal activity against Aedes aegypti(Linnaeus,1762)(Diptera:Culicidae)  

作　　者：Aileen Gonzalez Rizo Camilo E Castañet Martinez Celeste Ramírez Cardentey Ariamys Companioni Ibañez Zulema Menéndez Díaz Lianet Monzote Fidalgo Hilda M HernandezÁlvarez 

机构地区：[1]Vector Control Department,Institute of Tropical MedicinePedro Kourí,AutopistaNovia del Mediodía km 6½,La Habana 11400,Cuba [2]Virology Department,Institute of Tropical MedicinePedro Kourí,Autopista Novia del Mediodía km 6½,La Habana 11400,Cuba [3]Parasitology Department,Institute of Tropical MedicinePedro Kourí,Autopista Novia del Mediodía km 6½,La Habana 11400,Cuba

出　　处：《Asian Pacific Journal of Tropical Medicine》2022年第2期79-86,共8页亚太热带医药杂志（英文版）

摘　　要：Objective:To evaluate 11 Cuban native Bacillus(B.)thuringiensis isolates in order to select one with the best larvicidal activity against Aedes(Ae.)aegypti and low cytotoxicity.Methods:The cry and cyt genes of the isolates(A21,A51,L95,L910,M29,R84,R85,R87,R89,U81 and X48)were amplified by PCR.The influence of organic matter and NaCl on the larvicidal activity was tested by bioassays.Cytotoxicity was assayed on peritoneal macrophages of BALB/c mice.Results:The cyt1(Aa,Ab,Ba),cyt2,cry4aA,cry4Ba,cry11(Aa,Ba,Bb)and cry10 genes were identified in all native Cuban isolates.The larvicidal activity(LC_(90))of seven isolates was affected by the presence of organic matter in the water,while A21,A51,L910,R84,U81 and X48 had better LC_(50),LC_(90),LC_(95) than the 266/29-Ⅶ-98 control strain.The LC_(50) of two isolates was affected by the presence of NaCl and A21,A51,R85 isolate had better larvicidal activity than the 266/29-Ⅶ-98 control strain.In terms of toxicity against macrophages,the extracts of nine isolates were less cytotoxic than the control strains.Conclusions:Native isolate A21 had the main virulence factors against Ae.aegypti larvae,displayed a good larvicidal activity in presence of different factors related with Ae.aegypti breeding sites,and had low citotoxicity against macrophages.These results can contribute to the improvement of existing biological control strategies and the development of new biolarvicides.

关 键 词：MOSQUITOES Biological control agent Bacillus thuringiensis BIOASSAYS Aedes aegypti cry and cyt genes 

分 类 号：R38[医药卫生—医学寄生虫学]