基于SSR-HRM技术的向日葵DNA指纹图谱构建  

Establishment of DNA fingerprint for sunflower by SSR-HRM technique

作　　者：刘苡含 牟青山 陈珊宇[3] 阮关海[3] 胡晋[1,2] 关亚静 LIU Yihan;MOU Qingshan;CHEN Shanyu;RUAN Guanhai;HU Jin;GUAN Yajing(College of Agriculture and Biotechnology, Zhejiang University, Hangzhou 310058, China;Hainan Institute, Zhejiang University, Sanya 572025,Hainan,China;Institute of Crops and Nuclear Technology Utilization, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China)

机构地区：[1]浙江大学农业与生物技术学院,浙江杭州310058 [2]海南浙江大学研究院,海南三亚572025 [3]浙江省农业科学院作物与核技术利用研究所,浙江杭州310021

出　　处：《浙江农业学报》2022年第4期678-686,共9页Acta Agriculturae Zhejiangensis

基　　金：浙江省重点研发计划(2019C02004)。

摘　　要：以浙江省各地种植的32份向日葵种质为材料,利用17对简单重复序列标记引物结合高分辨率熔解曲线技术对32份材料进行遗传多样性分析与DNA指纹图谱构建。聚类分析结果表明,该32份材料遗传多样性相对较低,大部分材料的亲缘关系与其地理来源、所属类型基本一致。本研究创新了向日葵种质资源的鉴定方法,为进一步利用高分辨率熔解曲线技术鉴定向日葵种质资源奠定了基础。Combined with high-resolution melting curve analysis technology,17 pairs of core simple sequence repeats(SSR)primers were screened out for genetic diversity analysis and DNA fingerprint construction of 32 sunflower lines collected from Zhejiang Province.The results of cluster analysis showed that genetic diversity among the 32 materials was relatively low,and genetic relationships among most of 32 materials were consistent with their geographical sources and types.This study innovated the identification method of sunflower germplasm resources,and laid a foundation for further research on identification of sunflower germplasm by microsatellite high-resolution melting technique.

关 键 词：向日葵 SSR分子标记 高分辨率熔解曲线 指纹图谱 遗传多样性 

分 类 号：S565.5[农业科学—作物学]