四川省某奶牛场犊牛呼吸道疾病的细菌性病原qPCR检测  

qPCR detection of bacterial pathogens of respiratory diseases in calves of a dairy farm from Sichuan

作　　者：陈久兵 耿尚景超 王方国 陈娅婷 周金伟 骆巧 马莉 姚学萍[1] 余树民[1] 沈留红[1] 储岳峰[2] 曹随忠[1] CHEN Jiubing;GENGSHANG Jingchao;WANG Fangguo;CHEN Yating;ZHOU Jinwei;LUO Qiao;MA Li;YAO Xueping;YU Shumin;SHEN Liuhong;CHU Yuefeng;CAO Suizhong(College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China;State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou 730046, China;Agriculture and Rural Bureau of Pujiang County, Pujiang 611630, Sichuan, China)

机构地区：[1]四川农业大学动物医学院,四川成都611130 [2]中国农业科学院兰州兽医研究所,家畜疫病病原生物学国家重点实验室,甘肃兰州730046 [3]蒲江县农业农村局,四川蒲江611630

出　　处：《浙江农业学报》2022年第4期706-712,共7页Acta Agriculturae Zhejiangensis

基　　金：国家重点研发计划(2018YFD0502305);校企合作项目(002H2001)。

摘　　要：为了解四川省某规模化奶牛养殖场犊牛呼吸道疾病(bovine respiratory disease,BRD)的病原情况,进而制定防治措施,本研究通过TaqMan荧光定量PCR方法对2019—2020年75份有呼吸道疾病症状的犊牛鼻腔棉拭子和10份其他样品进行常见细菌性病原检测。结果显示,多杀性巴氏杆菌(Pm)、睡眠嗜血杆菌(Hs)、牛支原体(Mb)、化脓隐秘杆菌(Tp)和溶血性曼氏杆菌(Mh)的检出率分别为57.33%(43/75)、38.67%(29/75)、13.33%(10/75)、12.00%(9/75)和8.00%(6/75)。单一感染率为36.00%(27/75),主要由Pm引发,占24.00%(18/75);混合感染率为40.00%(30/75),尤以Hs和Pm引发的混合感染为主,占18.67%(14/75)。结果表明,5种常见BRD细菌性致病菌均有检出,且混合感染类型较多。Hs的高检出率表明其与我国BRD有一定联系,有必要在全国范围内进一步开展其流行病学调查。In order to explore the pathogens of bovine respiratory disease(BRD)in a large-scale dairy farm in Sichuan Province,TaqMan fluorescent quantitative PCR method was used to analyze 75 nasal cotton samples from the calves with respiratory disease symptoms and 10 other samples during 2019 to 2020 for common bacterial pathogens.The results showed that the detection rates of Pasteurella multocida(Pm),Histophilus somni(Hs),Mycoplasma bovis(Mb),Trueperella pyogenes(Tp)and Mannheimia haemolytica(Mh)were 57.33%(43/75),38.67%(29/75),13.33%(10/75),12.00%(9/75),8.00%(6/75).The single infection rate was 36.00%(27/75),mainly caused by Pm,accounting for 24.00%(18/75);the mixed infection rate was 40.00%(30/75),especially the mixed infection caused by Hs and Pm,accounted for 18.67%(14/75).The results showed that all five common BRD bacterial pathogens were detected,and there were many types of mixed infections.The high detection rate of Hs indicated that it had a certain connection with BRD in China,and it was necessary to carry out further epidemiological investigations nationwide.

关 键 词：牛呼吸道疾病 TaqMan荧光定量PCR 睡眠嗜血杆菌 混合感染 

分 类 号：S823[农业科学—畜牧学]