蚕豆耐盐相关基因VfHKT1;1的克隆、生物信息学分析及表达特性  

Cloning,bioinformatics analysis and gene expression pattern of VfHKT1;1 in Vicia faba L.

作　　者：樊有存 张红岩 杨旭升 韩芊 刘玉皎[1] 武学霞 FAN Youcun;ZHANG Hongyan;YANG Xusheng;HAN Qian;LIU Yujiao;WU Xuexia(State Key Laboratory of Plateau Ecology and Agriculture, Academy of Agricultural and Forestry, Qinghai University, Xining 810000, China;College of Eco-Environmental Engineering, Qinghai University, Xining 810000, China)

机构地区：[1]青海大学农林科学院省部共建三江源生态与高原农牧业国家重点实验室,青海西宁810000 [2]青海大学生态环境工程学院,青海西宁810000

出　　处：《浙江农业学报》2022年第4期756-765,共10页Acta Agriculturae Zhejiangensis

基　　金：青海省自然科学基金(2018-ZJ-940Q);青海大学省部共建三江源生态与高原农牧业国家重点实验室自主课题(2017-ZZ-12,2016-ZZ-04);国家食用豆产业技术体系建设专项(CARS-08)。

摘　　要：高亲和性钾离子转运蛋白(high affinity K+transporter,HKT)能够调节细胞及整株的Na+/K+转运,在植物盐胁迫调控中发挥着重要作用。采用RT-PCR和PCR方法克隆获得蚕豆VfHKT1;1基因全长编码序列,该基因包含1659 bp的开放阅读框,编码552个氨基酸。系统进化树分析发现,该蛋白属于HKT第Ⅰ亚家族,命名为VfHKT1;1,且VfHKT1;1蛋白氨基酸序列与苜蓿MtHKT1;1的序列相似度最高。生物信息学分析发现,VfHKT1;1蛋白结构稳定,不具有信号肽结构,含有8个跨膜区域,且蛋白含有HKT家族典型的保守结构域TrKH,属于典型的跨膜离子转运体。实时荧光定量PCR分析表明,VfHKT1;1基因的表达具有明显的组织特异性。在盐胁迫诱导下,VfHKT1;1基因在根中的表达水平受到抑制,而在叶片中的表达水平上升,且在盐处理1h后达到最高值,随后随着盐处理时间的延长又逐渐下降。High affinity K+transporter(HKT)involve in salt tolerance response through maintaining Na+/K+homeostasis in cellar and whole plant.VfHKT1;1 gene was cloned using RT-PCR and PCR in this work and the gene encoded an open reading frame(1659 bp)consisting of 552 amino acids.Phylogenetic tree analysis indicated that VfHKT1;1 belong to HKT subfamilyⅠand shared the highest sequence similarity with MtHKT1;1,so it was named as VfHKT1;1.The bioinformatics analysis showed that VfHKT1;1 was stable,which also contained 8 transmembrane regions and no signal peptide.It was supposed to be an ion transporter which usually located at membrane because it contains a typical TrKH conserved functional domain of HKT family.Real-time fluorescent quantitative PCR showed different expression levels of VfHKT1;1 gene in root and leaf.The expression of VfHKT1;1 gene in root was suppressed under salt stress.Whereas it had higher transcript levels in leaf under salt stress,the expression peak appeared at 1 h after NaCl treatment.Then,the expression level of VfHKT1;1 gene gradually declined with the NaCl treatment time.

关 键 词：蚕豆 基因克隆 生物信息学分析 表达特性 

分 类 号：S529[农业科学—作物学]