生物强度电场对人皮肤成纤维细胞转化的调节作用  

Regulatory effects of bio-intensity electric field on transformation of human skin fibroblasts

作　　者：王文平 冀然 张泽 邬亚婷 张恒术[1] 张琼 江旭品 滕苗[1] Wang Wenping;Ji Ran;Zhang Ze;Wu Yating;Zhang Hengshu;Zhang Qiong;Jiang Xupin;Teng Miao(Department of Plastic and Burn Surgery,the First Affiliated Hospital of Chongqing Medical University,Chongqing 400016,China;State Key Laboratory of Trauma,Burns and Combined Injury,Institute of Burn Research,the First Affiliated Hospital of Army Medical University(the Third Military Medical University),Chongqing 400038,China)

机构地区：[1]重庆医科大学附属第一医院整形烧伤外科,重庆400016 [2]陆军军医大学(第三军医大学)第一附属医院全军烧伤研究所,创伤、烧伤与复合伤国家重点实验室,重庆400038

出　　处：《中华烧伤与创面修复杂志》2022年第4期354-362,共9页Chinese Journal of Burns And Wounds

基　　金：国家自然科学基金面上项目(82072172);陆军军医大学科技创新能力提升专项(2020XQN12)。

摘　　要：目的探讨生物强度电场对人皮肤成纤维细胞(HSF)转化的调节作用。方法采用实验研究方法。取HSF,分为经200 mV/mm电场处理6 h的200 mV/mm电场组和置于电场装置中不通电处理6 h的模拟电场组,在活细胞工作站中观察细胞形态和排列变化;记录处理0、6 h细胞数,并计算细胞数变化率;观察并计算3 h内细胞运动方向、位移速度、轨迹速度(以上实验模拟电场组样本数为34、200 mV/mm电场组样本数为30);采用免疫荧光法检测处理3 h细胞α平滑肌肌动蛋白(α-SMA)的蛋白表达(样本数为3)。取HSF分为置于电场装置中不通电处理3 h的模拟电场组和经相应强度电场处理3 h的100 mV/mm电场组、200 mV/mm电场组、400 mV/mm电场组,另取HSF分为置于电场装置中不通电处理6 h的模拟电场组和经200 mV/mm电场处理相应时间的电场处理1 h组、电场处理3 h组、电场处理6 h组,采用蛋白质印迹法检测α-SMA、增殖细胞核抗原(PCNA)的蛋白表达(样本数为3)。对数据行Mann-Whitney U检验、单因素方差分析、独立样本t检验及LSD检验。结果处理6 h,与模拟电场组相比,200 mV/mm电场组细胞形态拉长,并产生局部粘连;模拟电场组细胞任意排列,200 mV/mm电场组细胞呈有规律的纵向排列;2组细胞数变化率相近(P>0.05)。处理3 h内,200 mV/mm电场组细胞有明显的向正极运动趋势,模拟电场组细胞绕原点运动;与模拟电场组比较,200 mV/mm电场组细胞位移速度和轨迹速度均明显加快(Z值分别为-5.33、-5.41,P<0.01),方向性显著增强(Z=-4.39,P<0.01)。处理3 h,200 mV/mm电场组细胞α-SMA蛋白表达较模拟电场组明显增加(t=-9.81,P<0.01)。处理3 h,100 mV/mm电场组、200 mV/mm电场组、400 mV/mm电场组细胞α-SMA蛋白表达分别为1.195±0.057、1.606±0.041、1.616±0.039,均明显多于模拟电场组的0.649±0.028(P<0.01)。与100 mV/mm电场组比较,200 mV/mm电场组、400 mV/mm电场组细胞α-SMA蛋白表达均明显�Objective To investigate the regulatory effects of bio-intensity electric field on the transformation of human skin fibroblasts(HSFs).Methods The experimental research methods were used.HSFs were collected and divided into 200 mV/mm electric field group treated with 200 mV/mm electric field for 6 h and simulated electric field group placed in the electric field device without electricity for 6 h.Changes in morphology and arrangement of cells were observed in the living cell workstation;the number of cells at 0 and 6 h of treatment was recorded,and the rate of change in cell number was calculated;the direction of cell movement,movement velocity,and trajectory velocity within 3 h were observed and calculated(the number of samples was 34 in the simulated electric field group and 30 in 200 mV/mm electric field group in the aforementioned experiments);the protein expression ofα-smooth muscle actin(α-SMA)in cells after 3 h of treatment was detected by immunofluorescence method(the number of sample was 3).HSFs were collected and divided into simulated electric field group placed in the electric field device without electricity for 3 h,and 100 mV/mm electric field group,200 mV/mm electric field group,and 400 mV/mm electric field group which were treated with electric fields of corresponding intensities for 3 h.Besides,HSFs were divided into simulated electric field group placed in the electric field device without electricity for 6 h,and electric field treatment 1 h group,electric field treatment 3 h group,and electric field treatment 6 h group treated with 200 mV/mm electric field for corresponding time.The protein expressions ofα-SMA and proliferating cell nuclear antigen(PCNA)were detected by Western blotting(the number of sample was 3).Data were statistically analyzed with Mann-Whitney U test,one-way analysis of variance,independent sample t test,and least significant difference test.Results After 6 h of treatment,compared with that in simulated electric field group,the cells in 200 mV/mm electric field group were

关 键 词：皮肤 成纤维细胞 肌成纤维细胞 细胞增殖 细胞转化 生物强度电场 

分 类 号：R622[医药卫生—整形外科]