脓毒症患者肠道菌群多样性和结构变化分析  

Analysis of the diversity and structural changes of intestinal flora in patients with sepsis

作　　者：关岚[1] 周衡[1] 王聪[1] 刘志伟[1] 赵斌[1] Guan Lan;Zhou Heng;Wang Cong;Liu Zhiwei;Zhao Bin(Department of Emergency Medicine,Beijing Jishuitan Hospital,Beijing 100035,China)

机构地区：[1]北京积水潭医院急诊科,北京100035

出　　处：《中华急诊医学杂志》2022年第4期514-519,共6页Chinese Journal of Emergency Medicine

摘　　要：目的探讨脓毒症患者发病过程中肠道菌群多样性和结构变化情况。方法对2015年11月至2016年3月期间于北京市多家三甲医院急诊科就诊并符合Sepsis3.0诊断标准的脓毒症患者25例和同时期30例非脓毒症患者的粪便样本进行检测,利用16S rRNA(16S ribosomal RNA)测序技术对肠道菌群进行测序,使用Uparse、Qiime、R及LEfSe软件等分析菌群多样性及其结构变化。结果脓毒症组和非脓毒症组患者在性别、年龄以及慢性基础疾病方面比较差异无统计学意义。两组在肠道菌群多样性方面比较差异无统计学意义(P>0.05)。两组在肠道菌群结构方面比较,差异有统计学意义(P<0.05),即脓毒症组患者肠道菌群中Negativicutes纲、Selenomonadales目、Veillonellaceae科、Lachnospiraceae科、Faecalibacterium属、Hafnia属、Lachnoclostridium属、Blautia属及Ruminococcus种的菌群丰度明显降低;而Bacilli纲、Coriobacteriia纲、Lactobacillales目、Coriobacteriales目、Clostridiaceae科、Coriobacteriaceae科、Clostridium_sensu_stricto属、Collinsella属及Collinsella_aerofaciens种的菌群丰度明显增加。结论脓毒症组与非脓毒症组患者相比较,肠道菌群多样性无改变;而脓毒症组的患者存在肠道菌群组成结构改变。Objective To explore the diversity and structural changes of intestinal flora in patients with sepsis.Methods A total of 55 patients were enrolled who visited the Emergency Department of several Grade III-A hospitals from November 2015 to March 2016.Among them,25 patients with sepsis metthe diagnostic criteria of Sepsis 3.0 and 30 patients had no sepsis.The intestinal flora from all fecal samples was sequenced by 16S rRNA sequencing technology.The diversity and structure changes of the flora were analyzed using Uparse,Qiime,R and LEfSe software.Results There was no significant difference in sex,age,chronic underlying diseases,and the diversity of intestinal flora between the sepsis and non-sepsis groups(all P>0.05).The structure of intestinal flora was significant different between the two groups(P<0.05).Abundances of Negativicutes,Selenomonadales,Veillonellaceae,Lachnospiraceae,Faecalibacterium,Hafnia,Lachnoclostridium,Blautia and Ruminococcus in intestinal flora from patients with sepsis were significantly reduced,but those of Bacilli,Coriobacteriia,Lactobacillales,Coriobacteriales,Clostridiaceae,Coriobacteriaceae,Clostridium_sensu_stricto,Collinsella and Collinsella_aerofaciens were significantly increased.Conclusions There is no change in the diversity of intestinal flora between the sepsis and non-sepsis groups.There are structural changes of intestinal flora in patients with sepsis.

关 键 词：脓毒症 肠道菌群 粪便 16S rRNA 

分 类 号：R459.7[医药卫生—急诊医学]