土牛膝UPLC特征图谱的研究  

Study on UPLC typical chromatogram of Achyranthes aspera

作　　者：方磊 胡亮 李瑞莲 王湘波 FANG Lei;HU Liang;LI Ruilian;WANG Xiangbo(Hunan Pharmaceutical Excipients Testing and Inspection Center,Hunan Engineering&Technology Research Center for Pharma-ceutical Quality Evaluation,Hunan Institute for Drug Control,Changsha,Hunan,410001 P.R.China)

机构地区：[1]湖南省药品检验研究院湖南药用辅料检验检测中心湖南省药品质量评价工程技术研究中心,湖南长沙410001

出　　处：《华西药学杂志》2022年第2期176-180,共5页West China Journal of Pharmaceutical Sciences

基　　金：湖南省自然科学基金资助项目(2020JJ9036)。

摘　　要：目的采用UPLC色谱法建立土牛膝特征图谱。方法以乙腈-0.1%磷酸溶液为流动相进行梯度洗脱,流速为0.3 mL·min^(-1),检测波长为250 nm。结果土牛膝3个不同基原共有峰共有3个,分别为β-蜕皮甾酮、25R牛膝甾酮、25S牛膝甾酮。结论所建UPLC特征图谱方法能有效区分土牛膝、广东土牛膝、川牛膝,但无法区分野生牛膝、粗毛牛膝、柳叶牛膝3个不同基原。OBJECTIVE To estabilish a UPLC chromatographic method of Achyranthes aspera L..METHODS Acetonitrile-0.1%formic acid solution was used as mobile phase for gradient elution.The flow rate was 0.3 mL·min^(-1)and the detection wavelength was 250 nm.RESULTS UPLC fingerprint results showed that there were three common peaks in three different primordia of Achyranthes.They were identified as beta-ecdysterone,25R-achyranthone and 25S-achyranthone,respectively.CONCLUSION The UPLC method can effectively distinguish A.aspera from Eupatorium Chinese L.and Cyathula officinalis Kuan,but it is impossible to distinguish three different primordia of A.bidentata(wild),A.aspera L.and A.longifolia(Makino).

关 键 词：土牛膝 野生牛膝 柳叶牛膝 广东土牛膝 川牛膝 超高效液相色谱法 三萜皂苷 质量评估 

分 类 号：R917[医药卫生—药物分析学]