枳壳理气质量标志物的有效性验证  

Validation of Q-markers for Regulating-qi in Aurantii Fructus

作　　者：钟民勇 庹福临 乔日发 杨林 杨武亮 袁金斌 ZHONG Minyong;TUO Fulin;QIAO Rifa;YANG Lin;YANG Wuliang;YUAN Jinbin(Key Lahoratoiy of Modern Preparation of Traditional Chinese Medicine,Ministry of Education,Jiangxi University of Chinese Medicine,Nanchang 330004 Jiangxi,China;Undergraduate of Research Practice Class,School of Pharmacy,Jiangxi University of Chinese Medicine,Nanchang 330006 Jiangxi,China)

机构地区：[1]江西中医药大学现代中药制剂教育部重点实验室,江西南昌330004 [2]江西中医药大学药学院中药学科研实践班,江西南昌330006

出　　处：《中药新药与临床药理》2022年第3期281-286,共6页Traditional Chinese Drug Research and Clinical Pharmacology

基　　金：国家自然科学基金项目(81860698,81760680)。

摘　　要：目的验证枳壳理气质量标志物(Q-marker)的有效性。方法采用离体肠肌实验,应用BL-420N信息化生物信号采集与分析系统,观察记录枳壳醇提物、水提物和Q-marker混合溶液对正常小鼠离体小肠平滑肌收缩活动的影响。在体实验将KM小鼠50只随机分为5组,即空白组、阳性药组、枳壳醇提物组、枳壳水提物组、Q-marker混合溶液组,分别按25 mL·kg^(-1)灌胃给药,连续7 d,采用炭末推进法,测定小肠推进率和胃排空率来评价各组的药效。结果离体实验中,枳壳醇提物组、枳壳水提物组和Q-marker混合溶液组在累积给药体积为50μL和150μL时,对正常小鼠离体小肠平滑肌收缩活动均呈现促进作用,而在累积给药体积350μL时,则呈现抑制作用。在体实验中,枳壳醇提物组、枳壳水提物组与Q-marker混合溶液组对正常小鼠胃排空和小肠推进均具有显著的促进作用(P<0.01),且枳壳醇提物组、枳壳水提物组与Q-marker混合溶液组之间无显著性差异。结论枳壳Q-marker混合溶液与提取物的理气作用无显著性差异,提示该研究确定的15个枳壳理气质量标志物能够整体上代表枳壳的质量属性。Objective To validate the effectiveness of Q-marker of regulating-qi in Aurantii fructus.Methods The isolated intestinal muscle experiment was used for in vitro experiments. BL-420 N information biological signal acquisition and analysis system was used to observe and record the effects of alcohol extract,water extract and Qmarker mixed solution of Aurantii fructus on the contractile activity of isolated small intestinal smooth muscle of normal mice. For in vivo experiments,fifty KM mice were randomly divided into five groups:blank group,positive drug, alcohol extract, water extract and Q-marker mixed solution groups. They were administered by gavage at dosage of 25 mL·kg^(-1)for 7 days,the small intestinal propulsion rate and gastric emptying rate were measured by carbon powder propulsion method to evaluate the efficacy of each group.Results Results of the in vitro experiment showed that, when the cumulative administration volumes of alcohol extract, water extract and Q-marker mixed solution groups of Aurantii fructus reached 50 μL and 150 μL,the contractile activities of isolated small intestinal smooth muscle of normal mice in these groups were promoted;and the cumulative administration volumes at 350 μL of the drugs showed inhibitory effect. In the in vivo experiment, compared with the blank group, the alcohol extract,water extract and Q-marker mixed solution of Aurantii fructus groups had significant promoting effects on gastric emptying and intestinal propulsion of normal mice(P<0.01),and there was no significant difference between the alcohol extract, water extract and Q-marker mixed solution groups.Conclusion There was no significant difference in regulating-qi effect between Q-marker mixed solution and extract of Aurantii fructus,which indicated that the 15 regulating-qi Q-markers of Aurantii fructus determined by the research group in the early stage can represent the quality attributes of Aurantii fructus as a whole.

关 键 词：枳壳 理气 质量标志物 有效性 药效学 在体实验 离体实验 

分 类 号：R285.5[医药卫生—中药学]