大气PM_(2.5)对大鼠海马组织脑源性神经营养因子及其受体TrkB蛋白表达的影响  

Effect of atmospheric PM_(2.5) on the protein expression of brain-derived neurotrophic factor and its receptor TrkB in the hippocampus tissue of rats

作　　者：康惠文 宋倩 陈晨 石聪 李清钊 王梦迪 杨舒越 蒋守芳 KANG Hui-wen;SONG Qian;CHEN Chen;SHI Cong;LI Qing-zhao;WANG Meng-di;YANG Shu-yue;JIANG Shou-fang(School of Public Health,North China University of Science and Technology/Hebei Coal Mine Health and Safety Laboratory,Tangshan 063210,China)

机构地区：[1]华北理工大学公共卫生学院河北省煤矿卫生与安全实验室,唐山063210

出　　处：《环境卫生学杂志》2022年第3期166-169,176,共5页JOURNAL OF ENVIRONMENTAL HYGIENE

基　　金：国家级大学生创新创业训练计划项目(X2016013)。

摘　　要：目的探讨大气PM_(2.5)染毒对大鼠海马组织中脑源性神经营养因子(BDNF)及其受体TrkB相关蛋白表达的影响。方法采集大气PM_(2.5),制备混悬液。将48只SPF级雄性SD大鼠随机分为对照组和暴露组,每组8只,染毒组大鼠按照20 mg/kg气管滴注PM_(2.5)混悬液,对照组大鼠气管滴注等量生理盐水,每周1次,分别染毒1个月、3个月和6个月。采用免疫组化法测定大鼠海马组织中PKA蛋白表达,蛋白免疫印迹法测定大鼠海马组织中BDNF、TrkB、AKT和CREB蛋白表达水平。结果与相应对照组比较,3个月、6个月染毒组大鼠海马组织中PKA阳性细胞减少,BDNF、TrkB、AKT和CREB蛋白表达水平均明显降低,差异均有统计学意义(P<0.05)。结论大气PM_(2.5)染毒可降低大鼠海马组织中BDNF、TrkB、AKT、CREB和PKA蛋白表达水平。Objective To investigate the effect of atmospheric fine particulate matter(PM_(2.5)) exposure on the protein expression of brain-derived neurotrophic factor(BDNF)and its related proteins of tyrosine receptor kinase B(TrkB)in the hippocampus tissue of rats.Methods PM_(2.5) was collected to prepare a suspension.Forty-eight specific pathogen-free male Sprague-Dawley rats were randomly divided into control groups and exposure groups,with 8 rats in each group.Rats in the exposure groups were treated with 20 mg/kg PM_(2.5) suspension via intratracheal instillation,and rats in the control groups were treated with the same amount of normal saline.The treatments were performed once a week for 1 month,3 months,and 6 months,respectively.The protein expression of PKA in the hippocampal tissue of the rats was determined by immunohistochemistry,and the protein expression levels of BDNF,TrkB,AKT,and CREB in the hippocampal tissue of the rats were determined by Western blot.Results Compared with the corresponding control groups,the 3-and 6-month exposure groups had significantly decreased PKA positive cells in the hippocampus tissue of the rats and significantly decreased protein expression levels of BDNF,TrkB,AKT,and CREB(P<0.05).Conclusion Exposure to atmospheric PM_(2.5) can reduce the protein expression levels of BDNF,TrkB,AKT,CREB,and PKA in the hippocampus tissue of rats.

关 键 词：细颗粒物(PM_(2.5)) 海马组织 脑源性神经营养因子(BDNF) 环磷腺苷效应元件结合蛋白 丝/苏氨酸激酶 

分 类 号：R12[医药卫生—环境卫生学]