类志贺邻单胞菌感染对杂交鲟脾脏转录组的影响  

Transcriptome analysis of sturgeons spleen infected with Plesiomonas shigelloides

作　　者：刘妍罕 张明洋 曾茂芹 张飘 杨霞 杨颖[1] 程振涛 姜海波[1] 文明[1] LIU Yanhan;ZHANG Mingyang;ZENG Maoqin;ZHANG Piao;YANG Xia;YANG Ying;CHENG Zhentao;JIANG Haibo;WEN Ming(Key Laboratory of Animal Epidemiology and Veterinary Publitic Heath of Guizhou/Research CenteRof Engineering Technology foRAnimal Biological Products of Guizhou/College of Animal Science,Guizhou University,Guiyang 550025,China)

机构地区：[1]贵州大学动物科学学院/贵州省动物疫病与兽医公共卫生重点实验室/贵州省动物生物制品工程技术研究中心,贵阳550025

出　　处：《扬州大学学报（农业与生命科学版）》2022年第1期33-41,共9页Journal of Yangzhou University：Agricultural and Life Science Edition

基　　金：贵州省科技支撑计划项目(黔科合支撑[2016]2502号、黔科合支撑[2016]2511号、黔科合支撑[2017]2532号);贵州省科技平台及人才团队计划项目(黔科合平台人才[2018]5253号)。

摘　　要：为探究类志贺邻单胞菌感染杂交鲟脾脏转录组的变化情况,以常见病原菌类志贺邻单胞菌接种约1年龄杂交鲟,于接种后24h采集杂交鲟脾脏组织样本,提取组织总RNA,采用Ilumina HiSeq 4000^(TM)进行转录组测序,筛选杂交鲟脾脏差异表达免疫应答基因并进行GO功能分类和KEGG信号通路分析。结果表明:与正常对照组相比,感染组杂交鲟脾脏差异表达基因为14069个,其中上调基因为10099个,下调基因为3970个;GO分析发现,差异表达基因显著富集到免疫效应过程、小分子代谢过程、防御反应、肽链内切酶活性、G蛋白偶联受体活性、肽酶活性、胞外区、胞外区组成部分和细胞骨架等;KEGG分析发现,差异表达基因显著富集在RIG-I样受体信号通路、神经活性配体-受体相互作用、细胞外基质受体相互作用、Toll样受体信号通路、细胞黏附分子、细胞因子-细胞因子受体相互作用、精氨酸生物合成、蛋白质消化吸收、细胞溶质DNA传感途径等,其中直接参与免疫应答的信号通路为RIG-I样受体信号通路、Toll样受体信号通路和细胞溶质DNA传感途径。这一研究为深入探究杂交鲟抵御细菌感染的分子机制提供了良好基础。In order to explore the change of spleen transcriptome in sturgeons infected with pathogenic Plesiomonas shigelloides,the hybrid sturgeons about 360 d were infected by P.shigellas,and their spleen tissues were collected after 24 h of inoculation and used for extraction of mRNA,and transcriptome sequencing was performed using high-throughput sequencing technology and analyzed for GO function classification and KEGG signal pathway.The results showed that there were 14069 of differentially expressed genes with 10099 of up-regulated genes and 3970 of down-regulated genes in the sturgeon intestinal comparing with the control group.GO analysis found that significantly enriched sturgeons gut differentially expressed genes were mainly involved in immune system process,immune effect process and stimulation response.Significant enrichment of molecular functions mainly included:DNA binding,signal receptor activity,nucleic acid transcription factor activity.The cell components were mainly extracellular,extracellular components,cell membrane.KEGG analysis showed that the immune signaling pathways involved in differentially expressed genes in sturgeons intestinal were RIG-I-like receptor signaling pathway,Toll-like receptor signaling pathway and cytosolic DNA-sensing pathway.These results provide a good basis for further research on the molecular mechanism of the hybrid sturgeon against bacterial infection.

关 键 词：杂交鲟 脾脏 高通量测序 转录组 类志贺邻单胞菌 

分 类 号：S855.1[农业科学—临床兽医学]