活体小鼠微血管血流倒置双光子激光扫描显微镜检测方法的建立  

Establishment of in vivo mice inverted two-photon laser scanning microscope microvascular blood flow imaging

作　　者：刘皎 丛馨[2] 何其华 LIU Jiao;CONG Xin;HE Qi-hua(Center of Medical and Health Analysis,Peking University Health Science Center,Beijing 100083,China;Department of Physiology and Pathophysiology,Peking University School of Basic Medical Sciences,Beijing 100083,China)

机构地区：[1]北京大学医药卫生分析中心生物成像与分析实验室,北京100083 [2]北京大学基础医学院生理学与病理生理学系,北京100083

出　　处：《解剖学报》2022年第2期261-265,共5页Acta Anatomica Sinica

基　　金：国家自然科学基金(31700898)。

摘　　要：目的 使用倒置双光子显微镜,建立连续及质量更好的活体微血管成像及血流计算。方法 将麻醉和尾静脉注射染料的小鼠放置于37℃热板中央,剥离的组织器官放置于打磨自制的低边缘共聚焦小皿内,生理盐水浸没,自制不同重量和大小的圆形金属垫片按压暴露的组织器官。利用上述改进的装置测量孕鼠(n=3)和正常雌鼠(n=3)的肝脏及后肢的微血管血流差异。结果 此方法可以实现稳定连续成像,孕鼠比正常雌鼠的肝脏及后肢血流速度更快。结论 此方法能对组织器官进行微血管三维立体成像和血流速度检测。Objective To establish a method of in vivo microvascular imaging and blood flow calculation with better continuous imaging quality. Methods Anesthetized mice with dye injection through tail vein were placed in the center of a 37 ℃ hot plate holder. The stripped tissues were placed in a self-made low edge confocal dish and immersed in normal saline. The exposed tissues were pressed with self-made circular metal pads of different weights and sizes. The microvascular blood flow in the liver and hind limb of pregnant mice(n=3) and normal female mice(n=3) were measured by the improved device. Results This method can accomplish stable and continuous imaging. The blood flow velocity of liver and hind limb of pregnant mice were faster than that of normal female mice. Conclusion This method can be used for three-dimensional imaging of microvessels and detection of blood flow velocity in organs.

关 键 词：微血管 活体成像 倒置双光子激光扫描显微术 血流检测 小鼠 

分 类 号：R331[医药卫生—人体生理学]