T淋巴细胞亚群和外周血B淋巴细胞内免疫抑制因子在传染性单核细胞增多症患儿中的表达  

Expression of T lymphocyte subsets and immunosuppressive factors in peripheral blood B lymphocytes in children with infectious mononucleosis

作　　者：杨杰[1] 丁芳[1] 苏丽[1] Yang Jie;Ding Fang;Su Li(Department of Clinical Laboratory,the Third People’s Hospital of Zhengzhou,Zhengzhou 450000,China)

机构地区：[1]河南省郑州市第三人民医院检验科,450000

出　　处：《中国实用医刊》2022年第3期42-46,共5页Chinese Journal of Practical Medicine

摘　　要：目的分析T淋巴细胞亚群和外周血B淋巴细胞内免疫抑制因子在传染性单核细胞增多症(IM)患儿中的表达意义。方法抽取2018年11月至2021年6月郑州市第三人民医院收治的IM患儿462例作为IM组,并以1∶1配比抽取同期健康体检儿童462例作为健康对照组。检测两组受试对象的T淋巴细胞亚群、细胞毒性T淋巴细胞相关抗原-4(CTLA-4)、程序性死亡受体-1(PD-1)、程序性死亡受体-1配体(PD-L1)水平。比较IM组与健康对照组T淋巴细胞亚群、外周血B淋巴细胞内免疫抑制因子水平,比较IM组中不同病情时期、不同异型淋巴细胞百分比(ALY%)患儿的T淋巴细胞亚群、外周血B淋巴细胞内免疫抑制因子水平,分析其相关性。结果IM组CD3^(+)、CD8^(+)、CTLA-4、PD-1、PD-L1水平高于健康对照组,CD4^(+)、CD4^(+)/CD8^(+)水平低于健康对照组(P<0.05)。IM组急性期CD3^(+)、CD8^(+)、CTLA-4、PD-1、PD-L1水平高于恢复期,CD4^(+)、CD4^(+)/CD8^(+)水平低于恢复期(P<0.05)。IM组中ALY%≥10%患儿的CD3^(+)、CD8^(+)、CTLA-4、PD-1、PD-L1水平高于ALY%<10%患儿,CD4^(+)、CD4^(+)/CD8^(+)水平低于ALY%<10%患儿(P<0.05)。Pearson分析结果显示,CD3^(+)(r=0.671、0.647)、CD8^(+)(r=0.685、0.598)、CTLA-4(r=0.694、0.612)、PD-1(r=0.671、0.712)、PD-L1(r=0.682、0.685)和病情时期、ALY%呈正相关(P<0.05),CD4^(+)(r=-0.469、-0.512)、CD4^(+)/CD8^(+)(r=-0.501、-0.567)和病情时期、ALY%呈负相关(P<0.05)。结论IM患儿机体T淋巴细胞亚群和外周血B淋巴细胞内免疫抑制因子异常表达,并与疾病时期、ALY%密切相关,可据此评估病情进展和严重程度,指导临床制定针对性治疗方案。Objective To analyze the expression significance of T lymphocyte subsets and immunosuppressive factors in peripheral blood B lymphocytes in children with infectious mononucleosis(IM).Methods A total of 462 children with IM treated in the Third People’s Hospital of Zhengzhou from November 2018 to June 2021 were selected as the IM group,and 462 healthy children who underwent physical examination during the same period were selected as the healthy control group with a ratio of 1∶1 with IM group.The levels of T lymphocyte subsets,cytotoxic T lymphocyte associated antigen-4(CTLA-4),programmed death receptor-1(PD-1),and programmed death receptor-1 ligand(PD-L1)of the two groups were measured.The levels of immunosuppressive factors in T lymphocyte subsets and peripheral blood B lymphocyte were compared between IM group and healthy control group,and the levels of immunosuppressive factors in T lymphocyte subsets and peripheral blood B lymphocyte in children with different disease stages and different percentages of allogeneic lymphocytes(ALY%)in IM group were compared,and the correlation was analyzed.Results Levels of cluster of differentiation(CD)3^(+),CD8^(+),CTLA-4,PD-1,and PD-L1 in IM group were higher than those in healthy control group,while CD4^(+)and CD4^(+)/CD8^(+)were lower than those in healthy control group(P<0.05).In IM group,levels of CD3^(+),CD8^(+),CTLA-4,PD-1 and PD-L1 in acute stage were higher than those in recovery stage,while CD4^(+)and CD4^(+)/CD8^(+)in acute stage were lower than those in recovery stage(P<0.05).In IM group,levels of CD3^(+),CD8^(+),CTLA-4,PD-1 and PD-L1 in children with ALY%≥10%were higher,and levels of CD4^(+)and CD4^(+)/CD8^(+)in children with ALY%≥10%were lower,compared with those in children with ALY%<10%(P<0.05).Pearson analysis showed that,CD3^(+)(r=0.671,0.647),CD8^(+)(r=0.685,0.598),CTLA-4(r=0.694,0.612),PD-1(r=0.671,0.712),PD-L1(r=0.682,0.685)were positively correlated with disease stage and ALY%(P<0.05);while CD4^(+)(r=-0.469,-0.512),CD4^(+)/CD8^(+)(r=-0.501,-0.56

关 键 词：传染性单核细胞增多症 T淋巴细胞亚群 外周血B细胞 免疫抑制因子 

分 类 号：R725.1[医药卫生—儿科]