茅苍术生品及麸炒品挥发油对脂多糖诱导人结肠上皮细胞炎症损伤的影响  

Effects of essential oil from crude and bran-processed Atractylodes lancea on LPS-induced inflammatory injury of HCoEpiC

作　　者：于艳[1] 贾天柱[1] 才谦[1] 吴振起[1] 龚伟[1] 袁媛[1] 樊晖[1] 于永铎[1] YU Yan;JIA Tian-zhu;CAI Qian;WU Zhen-qi;GONG Wei;YUAN Yuan;FAN Hui;YU Yong-duo(Liaoning University of Traditional Chinese Medicine,Shenyang 110847,China)

机构地区：[1]辽宁中医药大学,沈阳110847

出　　处：《中华中医药杂志》2022年第3期1374-1379,共6页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：国家自然科学基金项目(No.81803727);辽宁省博士启动基金项目(No.20180540125);辽宁省教育厅一般项目(No.L201906)。

摘　　要：目的:考察并比较茅苍术生品及麸炒品挥发油体外抗结肠炎的作用,基于IKB抑制蛋白激酶(IKK)/核转录因子κB(NF-κB)信号通路探讨其作用机制。方法:以脂多糖诱导人结肠上皮细胞炎症损伤模型,MTT法检测细胞活力,ELISA法检测炎症相关因子白细胞介素(IL)-4、IL-8、IL-12和肿瘤坏死因子-α(TNF-α)的水平;RT-PCR法检测IKKα、NF-κB、IL-4和IL-8 mRNA表达;Western blot法检测IKKα、p-IKKα、p-IKKβ、NF-κB蛋白表达量。结果:与模型组比较,茅苍术生品和麸炒品挥发油均能显著提高细胞的存活率(P<0.05);提高抑炎因子IL-4的水平(P<0.05),降低促炎因子IL-8、IL-12和TNF-α水平(P<0.05);提高IL-4 mRNA的水平(P<0.05),降低NF-κB和IL-8 mRNA水平(P<0.05);降低p-IKKα、p-IKKβ和NF-κB蛋白表达量(P<0.05);麸炒品的作用优于生品。结论:茅苍术生品及麸炒品挥发油具有明显体外抗结肠炎的作用,麸炒品的作用优于生品,其机制部分是通过调控IKK/NF-κB信号通路而发挥作用。Objective: To investigate and compare the effects of essential oil from crude and bran-processed Atractylodes lancea(AL) on colitis in vitro and its mechanism based on IKK/NF-κB signaling pathway. Methods: The inflammatory injury model of human colonic epithelial cells(HCoEpiC) by LPS was established. The cell viability was detected by MTT assay.The levels of interleukin(IL)-4, IL-8, IL-12 and tumor necrosis factor-α(TNF-α) were determined by ELISA, and the mRNA expressions of inhibitor of nuclear factor kappa-B kinase(IKK)α, nuclear factor-κB(NF-κB), IL-4 and IL-8 were determined by quantitative RT-PCR. The expressions of IKKα, p-IKKα, p-IKKβ, NF-κB proteins were determined by Western blot. Results: Compared with the model group, the cell viability were increased(P<0.05), and the production of IL-4was significantly increaed(P<0.05). But the productions of IL-8, IL-12 and TNF-αwere significantly decreaed(P<0.05).Furthermore, the mRNA expressions of IL-4 was significantly stimulated(P<0.05), but the mRNA expressions of IL-8 and NF-κB and the expressions of p-IKKα, p-IKKβ, NF-κB proteins were notably inhibited by essential oil obtained from both crude and bran-processed AL(P<0.05). The essential oil from the processed AL was more effective compared with that from crude AL.Conclusion: The volatile oil from crude and bran-processed AL have satisfactory effects on colitis in vitro, and the effect of branprocessed AL is better than that of crude AL. The anti-inflammatory effect of AL could partly be through IKK/NF-κB signaling pathway.

关 键 词：苍术 炮制 麸炒 溃疡性结肠炎 信号通路 脂多糖 人结肠上皮细胞 

分 类 号：R285[医药卫生—中药学]