基于PI3K/AKT信号通路探讨参茸肽对心肌肥厚大鼠的心肌保护作用  

Myocardial protective effect of Shenrong Peptide on cardiac hypertrophy rats based on PI3K/AKT signal pathway

作　　者：张壮 阚默 杨擎[1] 刘晓冉 明思彤 王继凤 林嘉楠[1] 李辉[2] 李娜 曲晓波[1] ZHANG Zhuang;KAN Mo;YANG Qing;LIU Xiao-ran;MING Si-tong;WANG Ji-feng;LIN Jia-nan;LI Hui;LI Na;QU Xiao-bo(Changchun University of Chinese Medicine,Changchun 130117,China;Qian Wei Hospital of Jilin Province,Changchun130012,China)

机构地区：[1]长春中医药大学,长春130117 [2]吉林省前卫医院,长春130012 [3]长春中医药大学人参科学研究院

出　　处：《中华中医药杂志》2022年第3期1662-1667,共6页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：吉林省科技发展项目(No.20200708056YY);吉林省人才开发资金资助项目(No.010500016)。

摘　　要：目的:基于PI3K/AKT信号通路探讨参茸肽对异丙肾上腺素(ISO)诱导心肌肥厚大鼠的心肌保护作用及分子机制。方法:将50只SD大鼠随机分为空白组,模型组,阳性组,参茸肽低、高剂量组,每组10只。按分组进行灌胃给药并皮下注射ISO 7 d建立心肌肥厚大鼠模型。观察大鼠一般状态;检测大鼠心电图;计算大鼠心脏系数;检测心肌细胞凋亡;心肌组织病理切片进行HE、Masson染色;检测大鼠血清中谷胱甘肽(GSH)、丙二醛(MDA)含量及超氧化物歧化酶(SOD)活力;检测大鼠心肌组织ATP含量;Western Blot法检测心肌组织中PI3K、AKT、PPARγ、IRS2、GLUT1和GLUT4蛋白表达水平。结果:参茸肽能够改善心肌肥厚大鼠的一般状态,使心电图ST段抬高。与模型组比较,参茸肽低、高剂量组大鼠心脏系数显著降低(P<0.05),参茸肽高剂量组心肌细胞总凋亡率显著降低(P<0.05)。HE染色显示:参茸肽可减轻心肌肥厚大鼠心肌损伤,改善大鼠心肌结构。Masson染色显示:参茸肽可抑制心肌肥厚大鼠心肌纤维化,与模型组比较,参茸肽低、高剂量组心肌组织CVF显著降低(P<0.01)。与模型组比较,参茸肽低、高剂量组血清中GSH含量和SOD活力显著升高(P<0.05,P<0.01),MDA含量显著降低(P<0.05,P<0.01),心肌组织中ATP含量显著升高(P<0.05,P<0.01),PI3K、AKT、PPARγ、IRS2、GLUT1和GLUT4蛋白相对表达水平显著升高(P<0.01,P<0.05)。结论:参茸肽对ISO诱导的心肌肥厚大鼠具有较好的心肌保护作用,其机制可能是通过激活PI3K/AKT信号通路,增加心肌细胞ATP,抑制心肌细胞凋亡,进而改善心肌肥厚。Objective: To investigate the myocardial protective effect and molecular mechanism of Shenrong Peptide on isoproterenol(ISO)-induced cardiac hypertrophy in rats based on PI3K/AKT signal pathway. Methods: A total of 50 SD rats were randomly divided into blank group, model group, positive group, low and high dose of Shenrong Peptide groups. Intragastric administration according to groups and the cardiac hypertrophy rat model was established by subcutaneous injection of ISO for 7 days. The general state of rats was observed;the electrocardiogram of rats was detected;the heart coefficient of rats was calculated;cardiomyocyte apoptosis was detected;the pathological sections of myocardial tissue was observed by HE and Masson staining;the content of glutathione(GSH) and malondialdehyde(MDA) and the activity of superoxide dismutase(SOD) in serum of rats were detected;the content of ATP in myocardial tissue of rats was detected;and the expression levels of PI3K, AKT,PPARγ, IRS2, GLUT1 and GLUT4 in myocardial tissue by were detected by Western Blot method. Results: Shenrong Peptide could improve the general state of rats with myocardial hypertrophy and elevate the ST segment of electrocardiogram. Compared with the model group, the heart coefficient of rats in low and high dose of Shenrong Peptide groups decreased significantly(P<0.05), and the total apoptosis rate of cardiomyocytes in high dose of Shenrong Peptide groups decreased significantly(P<0.05).HE staining showed that Shenrong Peptide could reduce myocardial injury and improve myocardial structure in rats with cardiac hypertrophy. Masson staining showed that Shenrong Peptide could inhibit myocardial fibrosis in rats with cardiac hypertrophy.Compared with the model group, the CVF of myocardial tissue in low and high dose of Shenrong Peptide groups decreased significantly(P<0.01). In low and high dose of Shenrong Peptide groups, the content of GSH and the activity of SOD in serum increased significantly(P<0.05, P<0.01), the content of MDA decreased significantly(P<0

关 键 词：参茸肽 异丙肾上腺素 心肌肥厚 PI3K/AKT信号通路 谷胱甘肽 丙二醛 超氧化物歧化酶 

分 类 号：R285.5[医药卫生—中药学]