电针对糖尿病周围神经痛大鼠脊髓背角缓激肽B1受体和磷酸化蛋白激酶C的影响  

Effects of electroacupuncture on kinin B1 receptor and phosphorylated protein kinase C in spinal cord dorsal horn in rats with diabetic peripheral neuropathic pain

作　　者：马益琪 胡群祺 邰昭霞 费雪瑜 陈卢杭 葛其亭 王涵芝 瞿思颖 蒋永亮[1,3,4] 邵晓梅 方剑乔[1,3,4] 何晓芬 MA Yi-qi;HU Qun-qi;TAI Zhao-xia;FEI Xue-yu;CHEN Lu-hang;GE Qi-ting;WANG Han-zhi;QU Si-ying;JIANG Yong-liang;SHAO Xiao-mei;FANG Jian-qiao;HE Xiao-fen(Third Clinical Medical College and Rehabilitation Medical College of Zhejiang Chinese Medical University,Hangzhou 310053,China;Shanghai University of Traditional Chinese Medicine,Shanghai 201203,China;Key Laboratory of Acupuncture and Neurology of Zhejiang Province.Hangzhou 310053,China;Institute of Acupuncture and Moxibustion,Zhejiang University of Traditional Chinese Medicine,Hangzhou 310053,China)

机构地区：[1]浙江中医药大学第三临床医学院,杭州310053 [2]上海中医药大学,上海201203 [3]浙江省针灸神经病学研究重点实验室,杭州310053 [4]浙江中医药大学针灸研究所,杭州310053

出　　处：《中华中医药杂志》2022年第3期1668-1671,共4页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：国家自然科学基金项目(N o.81804181,N o.81774389);浙江省自然科学基金项目(No.LQ17H270003);浙江省大学生创新创业训练计划(No.202010344015,No.202010344017);浙江省大学生科技创新活动计划暨新苗人才计划(No.2020R410012)。

摘　　要：目的:观察电针对糖尿病周围神经痛(DPNP)大鼠空腹血糖、热痛阈、脊髓背角缓激肽B1受体(B1R)和磷酸化蛋白激酶C(p-PKC)的影响。方法:高脂高糖饲养联合腹腔注射STZ构建DPNP大鼠模型,选取足三里穴、昆仑穴予以电针干预,每日1次,每次30min,持续7d。检测各组大鼠空腹血糖和热痛阈,Western Blot检测大鼠腰段脊髓背角B1R和p-PKC的蛋白表达水平。结果:第49天后,与正常组比较,模型组空腹血糖显著升高(P<0.01),热痛阈显著降低(P<0.01);电针干预后,与模型组比较,电针组热痛阈显著提高(P<0.01),B1R和p-PKC的蛋白表达显著下降(P<0.01)。结论:电针能显著下调DPNP大鼠脊髓背角B1R和p-PKC的蛋白表达,该作用可能参与电针缓解DPNP的过程。Objective: To observe the effects of electroacupuncture on fasting blood glucose, paw withdrawal latency,kinin B1 receptor(B1R) and phosphorylated protein kinase C(p-PKC) in spinal cord dorsal horn in rats with diabetic peripheral neuropathic pain(DPNP). Methods: The DPNP rat model was constructed by high-fat and high-sugar feeding combined with intraperitoneal injection of STZ, and electroacupuncture was applied to the Zusanli and Kunlun points once daily for 30 min,for 7 d. Measured fasting blood glucose and paw withdrawal latency in each group, and detected the protein expression of B1R and p-PKC in the dorsal horn of the lumbar spinal cord of rats by Western Blot. Results: After the 49th day, compared with the normal group, fasting blood glucose of the model group was significantly increased(P<0.01) and paw withdrawal latency was significantly reduced(P<0.01). After electroacupuncture intervention, compared with the model group, paw withdrawal latency was significantly higher(P<0.01) in the electroacupuncture group, and the protein expression of B1R and p-PKC was significantly decreased(P<0.01). Conclusion: Electroacupuncture could significantly down-regulate the protein expression of B1R and p-PKC in spinal cord dorsal horn in rats with diabetic peripheral neuropathic pain, which might be involved in the process of electroacupuncture relieving DPNP.

关 键 词：糖尿病周围神经痛 电针 脊髓背角 缓激肽B1受体 磷酸化蛋白激酶C 

分 类 号：R245[医药卫生—针灸推拿学]