三脉菝葜抗肿瘤活性组分化学成分研究  

Study on chemical constituents of anti-tumor components from Smilax trinervula

作　　者：梁永红[1] 侯奕 姚慧莹 苏明声[1] 余英才[1] 夏循礼[1] 黄慧莲[1] 齐麟[2] 赵海平[3] LIANG Yong-hong;HOU Yi;YAO Hui-ying;SU Ming-sheng;YU Ying-cai;XIA Xun-li;HUANG Hui-lian;QI Lin;ZHAO Hai-ping(Jiangxi University of Chinese Medicine,Nanchang 330004,China;Railway Police College,Zhengzhou 450053,China)

机构地区：[1]江西中医药大学,南昌330004 [2]铁道警察学院,郑州450053 [3]江西中医药大学中医学院

出　　处：《中华中医药杂志》2022年第3期1704-1709,共6页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：国家自然科学基金项目(No.81560640);江西省科技厅计划项目(No.20192ACB20016,No.20172BCB22010);江西省教育厅科学技术研究项目(No.GJJ190661);国家重点研发计划专项(No.2018YFC0807203)。

摘　　要：目的:研究三脉菝葜Smilax trinervula Miq.的抗肿瘤活性组分化学成分。方法:采用硅胶、Sephadex LH-20、反相ODS、AB-8型大孔吸附树脂等色谱方法以及半制备高效液相法进行分离纯化,根据化合物的理化性质及核磁共振等现代波谱技术进行结构鉴定。结果:从三脉菝葜抗肿瘤活性组分乙酸乙酯组分中分离得到16个化合物,分别鉴定为胡萝卜苷(1)、白皮杉醇(2)、纤细薯蓣皂苷(3)、薯蓣次苷B(4)、薯蓣皂苷元-3-O-β-D-吡喃葡萄糖苷(5)、反式白藜芦醇(6)、薯蓣皂苷(7)、isonarthogenin3-O-α-L-rhamnopyranosyl-(1→2)-O-[α-L-rhamnopyranosyl-(1→4)]-β-D-glucopyranoside(8)、原薯蓣皂苷(9)、槲皮素-3’-O-葡萄糖苷(10)、β-谷甾醇(11)、薯蓣皂苷元(12)、maakoline(13)、Gnetumontanin C(14)、scirpusin A(15)、resveratrol trans-dehydrodimer(16)。结论:化合物resveratrol trans-dehydrodimer、Gnetumontanin C、maakoline为首次从该属植物中分离得到,化合物scirpusin A、反式白藜芦醇为首次从该植物中分离得到。Objective: To investigate the anti-tumor chemical constituents of Smilax trinervula Miq.. Methods: The compounds were isolated and purified by column chromatography over normal phase silica gel, Sephadex LH-20, reversedphase ODS, AB-8 macroporous adsorption resin, and semi-preparative high performance liquid chromatography. The compound structures were identified by physicochemical properties and various spectroscopic analysis, including NMR. Results: Sixteen compounds were isolated from the anti-tumor active component, the ethyl acetate partion of Smilax trinervula. And their structures were identified as daucosterol(1), piceatannol(2), gracillin(3), prosapogenin B of dioscin(4), diosgenin-3-O-β-Dglucopyranoside(5), trans-resveratrol(6), dioscin(7), isonarthogenin 3-O-α-L-rhamnopyranosyl-(1→2)-O-[α-L-rhamnopyranosyl-(1→4)]-β-D-glucopyranoside(8), protodioscin(9), quercetin-3’-O-glucopyranoside(10), β-sitosterol(11), diosgenin(12),maakoline(13), Gnetumontanin C(14), scirpusin A(15), resveratrol trans-dehydrodimer(16). Conclusion: Compounds resveratrol trans-dehydrodimer, Gnetumontanin C and maakoline are obtained from Smilax genus for the first time. Compounds scirpusin A and trans-resveratrol were isolated from Smilax trinervula for the first time.

关 键 词：抗肿瘤活性 三脉菝葜 化学成分 二苯乙烯类 活性组分 

分 类 号：R284.1[医药卫生—中药学]