姜黄素对Tg2576转基因小鼠磷脂酰肌醇结合网格蛋白组装蛋白介导的神经元胞吞过程的作用  

Effects of cureumin on phosphatidylinositol-binding clathrin assembly mediated neuronal endocytosis in Tg2576 transgenic mice

作　　者：苏芮 刘密凤[1] 王玉贤[2] 马克信[3] 韩振蕴[4] SU Rui;LIU Mi-feng;WANG Yu-xian;MA Ke-xin;HAN Zhen-yun(Scientific Research Department,Beijing Hospital of Traditional Chinese Medicine,Capital Medical University,Beijing 100010,China;Department of Psychosomatic Medicine,Beijing Hospital of Traditional Chinese Medicine,Capital Medical University,Beijing 100010,China;Department of Neurology,Eye Hospital,China Academy of Chinese Medical Sciences,Beijing 100040,China;Shenzhen Hospital,Beijing University of Chinese Medicine,Shenzhen 518172,China)

机构地区：[1]首都医科大学附属北京中医医院科研处,北京100010 [2]首都医科大学附属北京中医医院身心医学科,北京100010 [3]中国中医科学院眼科医院神经内科,北京100040 [4]北京中医药大学深圳医院,深圳518172

出　　处：《中华中医药杂志》2022年第3期1719-1722,共4页China Journal of Traditional Chinese Medicine and Pharmacy

摘　　要：目的:研究姜黄素对Tg2576转基因小鼠磷脂酰肌醇结合网格蛋白组装蛋白(PICALM)介导的神经元胞吞过程的影响。方法:采用Morris水迷宫检测Tg2576转基因小鼠学习记忆功能,采用Western Blot方法检测小鼠海马PICALM、生长相关蛋白-43(GAP-43)以及Rab5的含量,采用ELISA检测海马β淀粉样蛋白[Aβ(1-40)]含量。结果:第4天模型组游泳路程显著高于正常组(P<0.05),姜黄素组与正常组差异无统计学意义。与正常组比较,模型组Ⅰ象限路径长占总路径的百分比和穿越平台次数显著降低(P<0.05),海马Aβ(1-40)、PICALM、Rab5显著升高(P<0.01,P<0.05),GAP-43表达显著降低(P<0.01);与模型组比较,姜黄素组小鼠穿越平台次数显著增多(P<0.05),PICALM表达显著降低(P<0.05),GAP-43表达显著升高(P<0.05)。结论:姜黄素很可能通过调节神经元的胞吞过程,增加神经元的可塑性,从而改善AD小鼠模型学习记忆功能。Objective: To study the effects of curcumin on phosphatidylinositol-binding clathrin assembly(PICALM)mediated neuronal endocytosis in Tg2576 transgenic mice. Methods: Morris water maze was used to detect the learning and memory function of Tg2576 transgenic mice. PICALM, GAP-43 and Rab5 in hippocampus of mice were detected by Western Blot, and Aβ(1-40) in hippocampus was detected by ELISA. Results: On the 4th day, the path length of model group was significantly higher than normal group(P<0.05), but there was no significant difference between curcumin group and normal group. Compared with normal group, the percentage of the length of quadrant Ⅰ path in total path and the times of crossing platform in model group were significantly lower(P<0.05), Aβ(1-40), PICALM and Rab5 in hippocampus of model group were significantly increased(P<0.01, P<0.05), GAP-43 was significantly decreased(P<0.01). Compared with model group, the times of crossing platform in curcumin group was significantly increased(P<0.05), the levels of PICALM was significantly decreased(P<0.05) and GAP-43 was significantly increased(P<0.05) in curcumin group. Conclusion: Curcumin may improve the learning and memory function of AD mice by regulating the endocytosis of neurons and increasing the plasticity of neurons.

关 键 词：姜黄素 神经元胞吞 磷脂酰肌醇结合网格蛋白组装蛋白 生长相关蛋白-43 

分 类 号：R285.5[医药卫生—中药学]