聚乙二醇-海藻酸钠水凝胶构建新型组织工程瓣膜支架  

A Novel Tissue-Engineered Heart Valve Scaffold Constructed by Poly(Ethylene Glycol)-Sodium Alginate Hydrogel

作　　者：徐旭[1] 洪昊[1] 乔韡华 史嘉玮[1] 董念国[1] Xu Xu;Hong Hao;Qiao Weihua(Department of Cardiovascular Surgery Union Hospital,Tongji Medical College,Huazhong University of Science and Technology Wuhan 430022,China)

机构地区：[1]华中科技大学同济医学院附属协和医院心脏大血管外科,武汉430022

出　　处：《华中科技大学学报（医学版）》2022年第2期203-206,共4页Acta Medicinae Universitatis Scientiae et Technologiae Huazhong

基　　金：国家自然科学基金资助项目(No.81873502)。

摘　　要：目的利用聚乙二醇-海藻酸钠水凝胶交联去细胞瓣膜构建组织工程瓣膜支架,并研究其生物学和生物力学性能。方法由聚乙二醇-海藻酸钠水凝胶交联去细胞瓣膜制备复合支架瓣膜,由戊二醛与瓣膜交联制备戊二醛交联瓣膜,通过组织学染色,扫描电镜,细胞增殖实验,生物力学测试研究复合支架瓣膜的生物学以及生物力学性能,并与戊二醛交联瓣膜进行比较。结果组织学染色显示去细胞瓣膜表面能够紧密结合聚乙二醇-海藻酸钠水凝胶。大体观察结果显示聚乙二醇-海藻酸钠水凝胶不会对去细胞瓣膜的外观产生明显变化。扫描电镜结果显示复合支架瓣膜比戊二醛交联瓣膜表面具有更多粗糙的微结构。细胞增殖实验表明复合支架比戊二醛更有利于细胞的生长和增殖。力学性能试验表明复合支架瓣膜的最大应变较戊二醛交联瓣膜显著增大。结论聚乙二醇-海藻酸钠水凝胶交联去细胞瓣膜构建的复合组织工程瓣膜具有良好的生物学和生物力学性能,比目前临床上使用的戊二醛交联瓣膜生物学性能更好,具有成为下一代瓣膜替代物的潜力。Objective To construct a novel tissue-engineered heart valve scaffold by using poly(ethylene glycol)-sodium alginate hydrogel and decellularized heart valve,and to investigate its biological and biomechanical properties.Methods The hybrid scaffold group was established by poly(ethylene glycol)-sodium alginate hydrogel and decellularized heart valve.And the glutaraldehyde group was made by heart valve crosslinked with glutaraldehyde.Histological staining,scanning electron micrographic observation,cell proliferation assay and biomechanical property test were performed to investigate the biological and biomechanical properties of the two groups.A comparison was made between two groups.Results Histological staining showed that poly(ethylene glycol)-sodium alginate hydrogel was tightly bonded with the surface of decellularized heart valve.General observation showed that there was no significant change in the appearance of the hybrid scaffold group.Scanning electron micrographic result showed that the surface of the hybrid scaffold group had more rough microstructures than that of the glutaraldehyde group.Cell proliferation assay showed that the hybrid scaffold group was more beneficial to cell growth and proliferation than the glutaraldehyde group.Biomechanical property test showed that the hybrid scaffold group had better performance than the glutaraldehyde group in the ultimate strain.Conclusion The novel tissue-engineered heart valve scaffold consisted of poly(ethylene glycol)-sodium alginate hydrogel and decellularized heart valve had satisfying biological and biomechanical properties.And it has potential to become the next-generation heart valve substitutive material.

关 键 词：组织工程瓣膜 去细胞瓣膜 戊二醛瓣膜 聚乙二醇-海藻酸钠水凝胶 

分 类 号：R318.11[医药卫生—生物医学工程]