铁皮石斛原球茎富硒悬浮培养条件优化  

Conditions Optimization of Selenium-enriched Suspension Culture for Protocorms of Dendrobium officinale

作　　者：韩素菊[1] 梅闯 HAN Suju;MEI Chuang(School of Urban and Rural Planning and Construction,Mianyang Normal University,Sichuan Mianyang 621000,China)

机构地区：[1]绵阳师范学院城乡建设与规划学院,四川绵阳621000

出　　处：《中国农业科技导报》2022年第4期210-217,共8页Journal of Agricultural Science and Technology

基　　金：四川省教育厅项目(16ZB0317)。

摘　　要：通过对铁皮石斛原球茎富硒悬浮培养的生长指标及多糖、生物碱、总蛋白质、硒含量等指标进行评价,优化铁皮石斛富硒悬浮培养条件,为开发富硒铁皮石斛产品提供理论依据与物质基础。研究结果表明,优化的铁皮石斛原球茎富硒悬浮培养基为MS+2.0 mg·L^(−1)6-BA+0.50 mg·L^(−1) NAA+30 g·L^(−1)蔗糖+0.05 mg·L^(−1) Na_(2)SeO_(3),接种量为100 g·L^(−1),pH为5.8,继代周期为30 d,此时原球茎的硒含量最高,可达4.01 mg·kg^(−1),多糖含量为34.79%、生物碱含量为0.026%、总蛋白质含量为28.25%。试验成功建立了富硒铁皮石斛原球茎悬浮培养体系。In this study,the growth index,polysaccharide,alkaloid,total protein,selenium content and other indexes of Dendrobium candidum Procorms Se-enriched suspension culture were evaluated to optimize the conditions of Se-enriched suspension culture of Dendrobium candidum,providing theoretical basis and material basis for the development of Se-enriched Dendrobium candidum products.The results showed that the optimal Se-enriched suspension medium for protocorms of Dendrobium officinale was MS+2.0 mg·L^(−1)6-BA+0.50 mg·L^(−1) NAA+30g·L^(−1) sucrose+0.05 mg·L^(−1) Na_(2)SeO_(3),inoculation amount was 100 g·L^(−1),pH was 5.8.When the subculture period was 30 days,the selenium content of protocorms was up to 4.01 mg·kg^(−1),polysaccharide content was 34.79%,alkaloid content was 0.026%,and total protein content was 28.25%.The suspension culture system of selenium-enriched suspension culture for protocorms of Dendrobium officinale was established successfully.

关 键 词：铁皮石斛 富硒 原球茎 悬浮培养 

分 类 号：R282[医药卫生—中药学]