Nucleus-Encoded Thylakoid Protein,OsY3IP1,Confers Enhanced Tolerance to Saline and Alkaline Stresses in Rice  

作　　者：Hyeran MOON Young-Ah KIM Ryoung SHIN Chang-Jin PARK 

机构地区：[1]Department of Molecular Biology, Sejong University [2]Department of Bioresources Engineering, Sejong University [3]RIKEN Center for Sustainable Resource Science [4]Plant Engineering Research Institute, Sejong University

出　　处：《Rice science》2022年第3期225-236,共12页水稻科学（英文版）

基　　金：supported by the National Research Foundation of South Korea(Grant Nos.NRF-2020R1A2C1007778 and 2015K2A2A4000129)。

摘　　要：Abiotic stress confers serious damage to the photosynthetic machinery,often resulting in plant growth inhibition.Hypothetical chloroplast open reading frame 3(Ycf3)-interacting protein 1(Y3IP1)is a nucleus-encoded thylakoid protein and plays an essential role in the assembly of photosystem I.The full-length cDNA over-expresser(FOX)gene-hunting system is an approach using systemically generated gain-of-function mutants.Among the FOX-rice lines,a line CE175 overexpressing rice Y3IP1gene(Os Y3IP1)displayed less inhibition of root growth under saline(NaCl)stress.The expression of Os Y3IP1 was up-regulated under saline and alkaline(Na2CO3)stresses in the rice variety Kitaake.After saline and alkaline treatments,transgenic Kitaake overexpressing OsY3IP1-GFP(OsY3IP1-GFPox/Kit)displayed higher levels of chlorophyll content compared to Kitaake.Under the stress conditions,the maximum quantum yield of photosystem II photochemistry levels was higher in OsY3IP1-GFPox/Kit than in Kitaake.The increased tolerance conferred by OsY3IP1 overexpression correlated with reduced reactive oxygen species accumulation.Our data provide new insights into the possible role of OsY3IP1 in the pathway suppressing photooxidative damage under stress conditions.These features can be further exploited to improve saline and alkaline tolerances of rice plants in future.

关 键 词：ALKALINE reactive oxygen species RICE SALINE stress tolerance 

分 类 号：S511[农业科学—作物学]