OsABT,a Rice WD40 Domain-Containing Protein,Is Involved in Abiotic Stress Tolerance  

作　　者：CHEN Eryong SHEN Bo 

机构地区：[1]College of Life and Environmental Sciences,Hangzhou Normal University,Hangzhou 311121,China [2]Life School of Science and Technology,Henan Institute of Science and Technology,Xinxiang 453003,China

出　　处：《Rice science》2022年第3期247-256,共10页水稻科学（英文版）

基　　金：supported by the Major Program of the Zhejiang Province for Food Crop Breeding(Grant No.2016C02050-6);the Key Program of Hangzhou Agricultural Scientific Research(Grant No.20191203B08)。

摘　　要：Plant growth and crop productivity are severely affected by abiotic stress on a global scale.WD40 repeat-containing proteins play a significant role in the development and environmental adaptation of eukaryotes.In this study,OsABT,a stress response gene,was cloned from rice(Oryza sativa L.cv.Nipponbare).OsABT encodes a protein containing seven WD40 domains.Expression analysis revealed that the OsABT gene was first up-regulated and then down-regulated following treatment with abscisic acid(ABA)and NaCl,but was down-regulated when treated with PEG8000.Subcellular localization results showed that OsABT was located in the cytoplasm and nucleus of Arabidopsis roots.OsABT transgenic Arabidopsis showed significantly increased tolerance to ABA and salt stress during plant seedling development.However,the transgenic lines were more sensitive to drought stress.Moreover,OsABT can interact with OsABI2,a component of ABA signaling pathway.These results showed that OsABT plays a positive regulatory role in response to salt stress and a negative role in response to drought stress in Arabidopsis.

关 键 词：abscisic acid ARABIDOPSIS drought stress RICE salt stress WD40 domain-containing protein 

分 类 号：S511[农业科学—作物学]