温度及保幼激素类似物对烟蚜茧蜂羽化时间和羽化率的影响  

Effects of temperature and juvenile hormone analogs on the emergence time and rate of Aphidius gifuensis(Hymenoptera: Aphidiidae)

作　　者：白晶晶 顾钢[2] 赖荣泉 周挺[2] 米月娥 韩梦 张榜 BAI Jingjing;GU Gang;LAI Rongquan;ZHOU Ting;MI Yuee;HAN Meng;ZHANG Bang(State Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops,College of Plant Protection,Fujian Agriculture and Forestry University,Fuzhou 350002,China;Fujian Province Corporation of China National Tobacco Corporation,Fuzhou 350003,China)

机构地区：[1]福建农林大学植物保护学院,闽台作物有害生物生态防控国家重点实验室,福州市350002 [2]中国烟草总公司福建省公司,福州市350003

出　　处：《中国烟草学报》2022年第2期131-137,共7页Acta Tabacaria Sinica

基　　金：福建省自然科学基金面上项目(2017J01617,2020J01523);中国烟草总公司福建省公司项目(201735000027127,2021350000240017);中国国家留学基金项目(202008350060)。

摘　　要：【目的】研究不同温度及不同浓度保幼激素类似物对烟蚜茧蜂Aphidiusgifuensis蛹期羽化时间和羽化率影响,探寻能延迟蚜茧蜂蛹羽化时间且保证较高存活率的最佳温度及类似物组合,为烟蚜茧蜂规模化生产与释放提供参考。【方法】采用液浸法,以对照(10%丙酮)及保幼激素类似物处理烟蚜茧蜂蛹,将其放入相对湿度为75%±5%,光周期为(12L:12D)人工气候箱中不同温度(10℃、16℃、22℃、32℃、38℃)下,观察、记载其羽化成蜂数、僵蚜总数及羽化时间。【结果】(1)特定组合温度及保幼激素类似物可有效延迟烟蚜茧蜂蛹的羽化时间。10℃时,ZR-512(1000ng/μL)、22℃时,ZR-512(1000ng/μL)和ZR-777(200 ng/μL)处理后对其羽化时间分别可延迟80.00 h、40.00 h和32.00 h。但ZR-512(5000 ng/μL)处理后,随着温度升高,烟蚜茧蜂蛹羽化时间逐渐缩短。(2)温度及保幼激素类似物对烟蚜茧蜂蛹羽化率有明显影响:10℃时,ZR-512(1000ng/μL)处理后,其羽化率(80.00%)较高。高温对延迟烟蚜茧蜂蛹羽化时间和羽化率有负面影响。当温度在38℃时,所有烟蚜茧蜂蛹均无法羽化。【结论】在温度10℃时,保幼激素类似物ZR-512(1000ng/μL)处理后能有效延迟烟蚜茧蜂蛹羽化时间且其羽化率较高。因此,其在生产中规模化繁殖、保存烟蚜茧蜂有良好的推广应用价值。[Background] The aim of this study is to determine the effects of juvenile hormone analogs and temperature on the emergence rate and emergence time of Aphidius gifuensis pupa. The optimal combination of juvenile hormone analogs and temperature that can maintain a high survival rate and delay the emergence time of A. gifuensis pupa was obtained, in order to provide basis for the large-scale production and release of A. gifuensis. [Methods] Aphidius gifuensis were treated with control(10% acetone) and juvenile hormone analogue by liquid immersion, and then placed in an artificial climate chamber with relative humidity of 75%±5% and photoperiod of 12L:12D at different temperatures(10℃, 16℃, 22℃, 32℃, 38℃). The emergence time, total number of aphid mummie and number of adults were observed. [Results](1) The emergence time of A. gifuensis could be effectively delayed under specific combination of juvenile hormone analogs and temperature. Under the combinations of 10℃ + 1000 ng/μL ZR-512, 22℃ + 1000 ng/μL ZR-512 or 200 ng/μL ZR-777, the emergence time of A. gifuensis pupa were delayed by 80.00h, 40.00 and 32.00 h, respectively. However, after treated with ZR-512(5000 ng/μL), the emergence time was gradually decreased with the increase of temperature.(2) juvenile hormone analogs and temperature had a significant influence on the emergence rate of A. gifuensis pupa: Under the combination of 10℃+ 1000 ng/μL ZR-512,the emergence rate of A. gifuensis pupa was improved about 80.00%. The high temperature had a negative effect on the emergence rate and emergence time of A. gifuensis pupa. When the temperature was 38℃, none of the pupae emerged. [Conclusion] Under the combination of temperature 10℃ + juvenile hormone analogs of 1000 ng/μL ZR-512, the emergence time can be effectively delayed and emergence rate of A. gifuensis pupa can be increased significantly. Such combined treatment has a good application value in large-scale reproduction and preservation of A. gifuensis in production.

关 键 词：温度 保幼激素类似物 烟蚜茧蜂 羽化时间 羽化率 

分 类 号：S435.72[农业科学—农业昆虫与害虫防治] S476[农业科学—植物保护]