远红光补光对辣椒幼苗生长和非生物胁迫抗性的影响  

Effects of Supplemental Far-Red Light on Growth and Abiotic Stress Tolerance of Pepper Seedlings

作　　者：董桑婕 姜小春 王羚羽 林锐 齐振宇[2] 喻景权[1] 周艳虹[1] DONG SangJie;JIANG XiaoChun;WANG LingYu;LIN Rui;QI ZhenYu;YU JingQuan;ZHOU YanHong(Department of Horticulture,College of Agriculture and Biotechnology,Zhejiang University/State Agriculture Ministry Key Laboratory of Horticultural Plant Growth and Development,Hangzhou 310058;Agricultural Experimental Station,Zhejiang University,Hangzhou 310058)

机构地区：[1]浙江大学农业与生物技术学院/农业部园艺植物生长发育重点实验室,杭州310058 [2]浙江大学农业试验站,杭州310058

出　　处：《中国农业科学》2022年第6期1189-1198,共10页Scientia Agricultura Sinica

基　　金：浙江省重点研发计划(2018C02010);国家现代农业产业技术体系(CARS-24-B-01)。

摘　　要：【目的】研究补照适量远红光(FR)对辣椒幼苗生长发育和非生物胁迫抗性的调控作用,旨在为实际生产过程中利用精确的光环境调控手段培育壮苗提供理论依据。【方法】以辣椒‘博辣红帅’品种为研究材料,将苗龄 7 d 的辣椒幼苗置于LED 光源对照光谱(NL;红 R/蓝 B=3/1,光量子通量密度 PPFD 为 150 μmol·m^(-2)·s^(-1))及在此基础上分别补充 10 μmol·m^(-2)·s^(-1)远红光(6% FR)、20 μmol·m^(-2)·s^(-1)远红光(13% FR)和 30 μmol·m^(-2)·s^(-1)远红光(20% FR)的处理组光谱环境条件下培养,并于苗龄 21 d 时进行低温和干旱处理。通过测定生物量、抗性相关基因表达、抗氧化酶活性、激素含量、叶绿素荧光参数以及叶片相对电导率等,探究补充 6% FR 对辣椒幼苗生长和非生物胁迫抗性的影响。【结果】与对照光谱相比,补充 6% FR 显著提高了辣椒幼苗株高、茎粗、干鲜重以及壮苗指数(P<0.05)。低温胁迫下,相比于对照光谱组,补充 6% FR 显著提高了辣椒叶片冷响应基因 CBF1 和抗氧化酶相关基因 Cu/Zn-SOD、GR、APX、CAT、DHAR 的表达水平,超氧化物歧化酶(SOD)、抗坏血酸过氧化物酶(APX)、脱氢抗坏血酸还原酶(DHAR)、过氧化氢酶(CAT)、谷胱甘肽还原酶(GR)活性比对照分别增加 25.2%、53.6%、55.8%、72.7%和 33.4%,抗逆相关激素脱落酸(ABA)的含量提高 69.5%。同时,低温胁迫下补充 6% FR 后辣椒叶片PSII 最大光化学效率(Fv/Fm)较对照光谱组显著升高,而相对电导率(REL)显著降低,表明补充 6% FR 缓解了低温下 PSII光抑制和叶片细胞的损伤,提高了辣椒幼苗的耐冷性。此外,干旱胁迫下,相比于对照光谱组,补充 6% FR 使辣椒幼苗的抗氧化酶 SOD、GR、APX、CAT、DHAR 活性分别增加 13.7%、38.0%、37.2%、27.6%和 23.7%,ABA 含量和 PSII 实际光化学效率(Φ;)也显著升高,而 REL 则明显降低,表明补充 6% FR 减轻了干旱胁迫引起的 PSII 光抑制和膜�【Objective】This study analyzed the effects of supplementary far-red light (FR) on the growth and abiotic stress tolerance of pepper seedlings, aiming to provide a theoretical basis on precise light environments for cultivating high quality vegetable seedlings.【Method】In this study, Bola Hongshuai pepper cultivar was used as the research material. The 7-day-old seedlings were cultivated under two LED light environments, including the control spectrum (NL;R/B = 3/1, 150 μmol·m^(-2)·s^(-1)PPFD) and the NL with an extra 10 μmol·m^(-2)·s^(-1)far-red light (6% FR), 20 μmol·m^(-2)·s^(-1)far-red light (13% FR), and 30 μmol·m^(-2)·s^(-1)far-red light (20% FR)Chilling and drought were imposed when the seedlings were 21 days old. Biomass, resistance-related gene expression, antioxidant enzyme activity, hormone content, chlorophyll fluorescence parameters, and leaf relative electrolyte leakage (REL) were analyzed to explore the effects of supplemental FR on growth and abiotic stress tolerance of pepper seedlings. 【Result】 Compared with the control, the supplementation of 6% FR was beneficial to increase the height, stem thickness, dry weight, fresh weight and seedling indexes of pepper seedlings. Moreover, the supplementation of 6% FR significantly increased the expression of the cold response gene CBF1 and antioxidant enzyme-related genes, such as Cu/Zn-SOD, GR, APX, CAT and DHAR under chilling stress. The activity of SOD, APX, DHAR, CAT and GR as well as the ABA content of pepper seedlings under low temperature increased by 25.2%,53.6%, 55.8%, 72.7%, 33.4% and 69.5%, respectively, following the treatment with supplemental FR. The PSII maximum photochemical efficiency (Fv/Fm) of pepper leaves after supplementation of 6% FR under low temperature stress significantly increased compared with the control, while REL decreased obviously, indicating that supplementation of 6% FR alleviated the low temperature-induced PSII photoinhibition and damage in leaves and enhanced the cold tolerance of pepper seedlin

关 键 词：辣椒 远红光 壮苗指数 低温胁迫 干旱胁迫 

分 类 号：S641.3[农业科学—蔬菜学]