BANF2基因在大鼠睾丸不同发育时期的表达及初步研究  

The expression of BANF2 gene in different developmental stages of rat testis and preliminary study

作　　者：韦龙生 杨晓芳 李慧 Wei Longsheng;Yang Xiaofang;Li Hui(Department of physiology,Fenyang College of Shanxi Medical University,Fenyang,Shanxi,032200,China)

机构地区：[1]山西医科大学汾阳学院,山西汾阳032200

出　　处：《齐齐哈尔医学院学报》2022年第4期304-307,共4页Journal of Qiqihar Medical University

基　　金：吕梁市重点研发项目(社会发展领域)(2021SHFZ-2-100)。

摘　　要：目的 观察大鼠睾丸中BANF2基因在不同发育时期的表达,进而研究该基因在精子发生中可能起的作用。方法 取三组出生后2-65日龄总计21个时间点的Wistar雄性大鼠,从各时间点的睾丸组织中提取总mRNA和总蛋白,并用实时定量PCR、Western blot技术检测BANF2基因在大鼠精子发生过程的表达水平,免疫组化观察BANF2蛋白在60日龄大鼠睾丸生精小管中的分布。结果 2-31日龄的大鼠睾丸组织mRNA表达极少,从35日龄mRNA表达开始明显增加,一直到65日龄。20-65日龄大鼠睾丸组织中BANF2蛋白表达要比2-12日龄和14-16日龄的蛋白表达显著增高。BANF2蛋白主要分布在生精小管中的圆形精子细胞和长形精子细胞。结论 BANF2基因主要参与精子成熟过程,该蛋白主要分布于圆形精子细胞和长形精子细胞,可能参与精子细胞的变形过程。Objective To observe the expression of BANF2 gene in rat testis at different developmental stages, and to study the possible role of this gene in spermatogenesis. Methods Three groups of male Wistar rats aged 2-65 days after birth were collected at 21 time points. Total mRNA and total protein were extracted from the testis tissues at each time point, and the BANF2 gene was detected by real-time quantitative PCR and Western blot. The immunohistochemical method was used to observe the distribution of BANF2 protein in the seminiferous tubules of 60-day-old rat testis.Results The expression of mRNA in the testis tissue of rats aged 2-31 days was at a very low level, and the mRNA expression began to increase significantly from the age of 35 days until the age of 65 days. The expression of BANF2 protein in the testis tissues of rats aged 20-65 days was significantly higher than those of 2-12 days and 14-16 days. BANF2 protein is mainly distributed in round spermatids and elongated spermatids in seminiferous tubules. Conclusions BANF2 gene is mainly involved in the process of sperm maturation. The protein is mainly distributed in round sperm cells and elongated sperm cells, it may be involved in the deformation process of sperm cells.

关 键 词：BANF2 精子发生 大鼠睾丸 精子成熟 

分 类 号：R339.2[医药卫生—人体生理学]