单增李斯特菌生物被膜形成不同时段sRNA的筛选  

Screening of sRNA in different time intervals of Listeria monocytogenes biofilm

作　　者：委慧玲 齐玉梅 张武宏 田常青 宋晨 李天昊 史文静 祁国军 魏衍全 薛惠文[1] 苟惠天[1] WEI Huiling;QI Yumei;ZHANG Wuhong;TIAN Changqing;SONG Chen;LI Tianhao;SHI Wenjing;QI Guojun;WEI Yanquan;XUE Huiwen;GOU Huitian(College of Veterinary Medicine,Gansu Agricultural University,Lanzhou 730070,China;Qingyang Animal Husbandry Technology Extension Center,Qingyang 745000,China)

机构地区：[1]甘肃农业大学动物医学院,甘肃兰州730070 [2]庆阳市畜牧技术推广中心,甘肃庆阳745000

出　　处：《畜牧与兽医》2022年第4期72-79,共8页Animal Husbandry & Veterinary Medicine

基　　金：国家自然科学基金项目(31960726,32060822,31560700);国家重点研发计划项目(2019YFC1605705);甘肃农业大学青年导师扶持基金(GAU-QDFC-2020-10)。

摘　　要：为了筛选与单增李斯特菌(LM)生物被膜形成相关的非编码小RNA(sRNA),并初步探索sRNA及其靶基因生物学功能,本研究通过高通量测序和实时荧光定量PCR比较LM浮游菌和生物被膜形成不同时段sRNA的差异情况,筛选与LM生物被膜形成相关的sRNA;预测sRNA的靶基因及分析其可能参与生物被膜形成的调节通路。结果表明:与LM浮游菌相比,预测到38个新的sRNA,其中sRNA00085、sRNA00019、sRNA00058在LM生物被膜形成中显著上调,sRNA00054、sRNA00081、sRNA00111显著下调;3个上调sRNA和3个下调sRNA的靶基因可能参与碳代谢、不同环境中微生物代谢、核糖体、糖代谢、氨酰基-tRNA生物合成及脂肪酸代谢6条调控通路,是潜在的LM生物被膜调控因子。研究结果为深入研究LM生物被膜sRNA调控机制提供了理论依据。In order to screen the sRNA related to the biofilm formation of Listeria monocytogenes, and to explore the biological function of sRNA and its target genes, high-throughput sequencing and quantitative Real-time PCR were used to compare the differences of sRNA between planktonic bacteria and biofilm of LM in different time intervals, and to screen the target genes related to LM biofilm formation and analyze the regulatory pathways through which sRNA might be involved in the biofilm formation. The results showed that, compared with the LM planktonic bacteria, 38 new sRNA were predicted, of which sRNA00085, sRNA00019 and sRNA00058 were significantly up-regulated in the LM biofilm, while sRNA00054, sRNA00081 and sRNA00111 were significantly down-regulated in the process. The target genes from the above sRNA might be involved in six regulatory pathways: carbon metabolism, microbial metabolism in different environments, ribosome, sugar metabolism, aminoacyl-tRNA biosynthesis and fatty acid metabolism, which were potential biofilm regulators of LM. The present results provided theoretical basis for the further study of the sRNA regulation mechanism of LM.

关 键 词：单核细胞增生李斯特菌 生物被膜 非编码小RNA 调控 

分 类 号：S855.1[农业科学—临床兽医学]