低总氮浓度下Fe^(2+)促进ANAMMOX生物膜反应器脱氮  

Promoting Nitrogen Removal in ANAMMOX Biofilm Reactor by Fe^(2+)Under Low Nitrogen Concentration

作　　者：郑旭文 秦嘉富 汪晓军 陈浩川[1,2] 朱梓键 陈振国 ZHENG Xu-wen;QIN Jia-fu;WANG Xiao-jun;CHEN Hao-chuan;ZHU Zi-jian;CHEN Zhen-guo(School of Environment and Energy,South China University of Technology,Guangzhou 510006,China;Key Laboratory of Pollution Control and Ecosystem Restoration in Industry Cluster,Ministry of Education,Guangzhou 510006,China;Hua An Biotech Co.,Ltd.,Foshan 528300,China;School of Chemistry and Chemical Engineering,South China University of Technology,Guangzhou 510641,China)

机构地区：[1]华南理工大学环境与能源学院,广州510006 [2]工业聚集区污染控制与生态修复教育部重点实验室,广州510006 [3]佛山市化尔铵生物科技有限公司,佛山528300 [4]华南理工大学化学与化工学院,广州510641

出　　处：《环境科学》2022年第4期2047-2054,共8页Environmental Science

基　　金：2019年广东省科技计划项目重点领域研发计划项目(2019B110205002);2020年高校教师特色创新研究项目(2020JNHB07)。

摘　　要：在低总氮(TN)浓度条件下考察了Fe^(2+)促进串联两级ANAMMOX生物膜反应器脱氮性能的可行性.结果表明,ρ(Fe^(2+))为5、10和15 mg·L^(-1)能够有效促进厌氧氨氧化反应,ρ(Fe^(2+))为10 mg·L^(-1)对两级ANAMMOX生物膜反应器的促进程度最大,在进水ρ(TN)约为150 mg·L^(-1),总氮负荷(NLR)为0.62 kg·(m^(3)·d)^(-1)条件下,最高总氮去除率(NRE)可达81.71%.添加Fe^(2+)可促进系统胞外聚合物(EPS)的分泌以及亚铁血红素c的合成.批次试验结果进一步验证了ρ(Fe^(2+))为5、10和15 mg·L^(-1)时对厌氧氨氧化菌活性的促进作用,其中ρ(Fe^(2+))为10 mg·L^(-1)时的比厌氧氨氧化活性(SAA)是对照组的3.6倍,而当ρ(Fe^(2+))为20 mg·L^(-1)时,AnAOB活性受到明显抑制.高通量测序结果显示,投加Fe^(2+)均促进了反应器中Candidatus_Kuenenia丰度的增加,其中ρ(Fe^(2+))为10 mg·L^(-1)时两个反应器中Candidatus_Kuenenia的相对丰度分别增至16.18%和4.22%.基于Fe^(2+)促进下两级厌氧氨氧化的稳定运行为厌氧氨氧化生物膜工艺处理低总氮浓度废水提供了参考.The feasibility for nitrogen removal in a two-stage ANAMMOX biofilm reactor promoted by Fe^(2+)under low nitrogen concentration was investigated.The results showed that the ANAMMOX reaction could be effectively promoted by aρ(Fe^(2+))of 5,10,and 15 mg·L^(-1).Aρ(Fe^(2+))of 10 mg·L^(-1) presented the highest promotion for the ANAMMOX reaction,with the highest nitrogen removal efficiency(NRE)of 81.71%under aρ(TN)of 150 mg·L^(-1)and a nitrogen loading rate(NLR)of 0.62 kg·(m^(3)·d)^(-1).Fe^(2+)promoted the secretion of extracellular polymeric substance(EPS)and the synthesis of heme c in the ANAMMOX system.Batch test results further verified the positive effects by Fe^(2+)on the activity of anaerobic ammonium oxidizing bacteria(AnAOB).The specific ANAMMOX activity(SAA)of 10 mg·L^(-1)ρ(Fe^(2+))was 3.6 times as high as that of the control group[ρ(Fe^(2+))=0 mg·L^(-1)],whereas the activity of AnAOB was significantly inhibited withρ(Fe^(2+))increased to 20 mg·L^(-1).High-throughput sequencing results showed that the addition of Fe^(2+)increased the abundance of Candidatus_Kuenenia.Whenρ(Fe^(2+))was 10 mg·L^(-1),the relative abundance of Candidatus_Kuenenia in reactor 1 and reactor 2 increased to 16.18%and 4.22%,respectively.The stable operation of the two-stage ANAMMOX biofilm process promoted by Fe^(2+)provides an alternative technology for low-strength nitrogen wastewater.

关 键 词：厌氧氨氧化(ANAMMOX) Fe^(2+) 生物膜 生物脱氮 促进作用 

分 类 号：X703[环境科学与工程—环境工程]