Epidural oscillating field stimulation increases axonal regenerative capacity and myelination after spinal cord trauma  

作　　者：Maria Bacova Katarina Bimbova Alexandra Kisucka Nadezda Lukacova Jan Galik 

机构地区：[1]Institute of Neurobiology of Biomedical Research Center,Slovak Academy of Sciences,Soltesovej,Kosice,Slovak Republic

出　　处：《Neural Regeneration Research》2022年第12期2730-2736,共7页中国神经再生研究（英文版）

基　　金：supported by grants of the Scientific Grant Agency of the Slovak Academy of Sciences:2/0098/20(JG);2/0145/21(NL);the Slovak Research and Development Agency:APVV19-0324(to NL);APVV-18-0163(MB);the Operational Programme Integrated Infrastructure for the project:Long-term strategic research of prevention, intervention and mechanisms of obesity and its comorbidities, IMTS:313011V344;co-financed by the European Regional Development Fund”(JG)

摘　　要：Oscillating field stimulation(OFS)with regular alterations in the polarity of electric current is a unique,experimental approach to stimulate,support,and potentially guide the outgrowth of both sensory and motor nerve fibers after spinal cord injury(SCI).In previous experiments,we demonstrated the beneficial effects of OFS in a 4-week survival period after SCI.In this study,we observed the major behavioral,morphological,and protein changes in rats after 15 minutes of T9 spinal compression with a 40 g force,followed by long-lasting OFS(50μA),over a 8-week survival period.Three groups of rats were analyzed:rats after T9 spinal compression(SCI group);SCI rats subjected to implantation of active oscillating field stimulator(OFS+SCI group);and SCI rats subjected to nonfunctional OFS(nOFS+SCI group).Histopathological analysis of spinal tissue indicated a strong impact of epidural OFS on the reduction of tissue and myelin loss after SCI in the segments adjacent to the lesion site.Quantitative fluorescent analysis of the most affected areas of spinal cord tissue revealed a higher number of spared axons and oligodendrocytes of rats in the OFS+SCI group,compared with rats in the SCI and nOFS+SCI groups.The protein levels of neurofilaments(NF-l),growth-associated protein-43(marker for newly sprouted axons),and myelin basic protein in rats were signifiantly increased in the OFS+SCI group than in the nOFS+SCI and SCI groups.This suggests a supporting role of the OFS in axonal and myelin regeneration after SCI.Moreover,rats in the OFS+SCI group showed great improvements in sensory and motor functions than did rats in the nOFS+SCI and SCI groups.All these findings suggest that long-lasting OFS applied immediately after SCI can provide a good microenviroment for recovery of damaged spinal tissue by triggering regenreative processes in the acute phase of injury.

关 键 词：axonal regenerative capacity behavioral assessment epidural stimulation motor recovery myelin regeneration oscillating field stimulation spinal cord injury 

分 类 号：R651.2[医药卫生—外科学]