间充质干细胞外泌体对CLP大鼠急性肾损伤作用研究  

The Effect of Mesenchymal Stem Cell-derived Exosomes on Acute Kidney Injury in CLP Rats

作　　者：徐莹[1,2,3] 周茹 张欣洲 马华林[1,2,3,4] XU Ying;ZHOU Ru;ZHANG Xin-zhou;MA Hua-lin(The Second Clinical Medical College,Jinan University;The First Affiliated Hospital,Southern University of Science and Technology;Shenzhen People′s Hospital;Shenzhen Key Laboratory of Nephrology,Shenzhen,Guangdong 518020,China)

机构地区：[1]暨南大学第二临床医学院 [2]南方科技大学第一附属医院 [3]深圳市人民医院 [4]深圳市肾脏病重点实验室,广东深圳518020

出　　处：《湖北医药学院学报》2022年第2期116-120,F0002,共6页Journal of Hubei University of Medicine

基　　金：深圳市肾脏病重点实验室项目(ZDSYS201504301616234);广东省高水平临床重点专科项目(SZGSP001);深圳市科技计划项目(JCYJ20180301170030277)。

摘　　要：目的:探究脐带间充质干细胞(human umbilical cord mesenchymal stem cells,HuMSCs)来源的外泌体(exosome)对脓毒症急性肾损伤大鼠的保护作用。方法:选取雄性SPF级SD大鼠30只随机分为对照组(Control组)、模型组(Model组)、MSC来源exosome处理组(Exosome组),每组10只。对比三组实验大鼠生存率、肾功能水平、血液炎性介质水平及肾组织增殖细胞核抗原(proliferating cell nuclear antigen,PCNA)增殖情况。结果:与Model组相比,Exosome组肾脏血肌酐、尿素氮、胱抑素C水平均有明显下降,差异有统计学意义(P<0.05);Exosome治疗6 d后,大鼠血清IL-6、TNF-α和IL-1β显著下降(P<0.05)。PCNA增殖检测显示Model组增殖上皮细胞增多,Exosome组增殖细胞增多更加明显。结论:脐带间充质干细胞来源的外泌体对脓毒症急性肾损伤大鼠有保护作用,可改善肾功能、降低炎性水平和提高肾组织细胞增殖水平。Objective To explore the protective effect of exosomes derived from human umbilical cord mesenchymal stem cells(HuMSCs)on acute kidney injury in rats with sepsis.Methods Thirty SPF male SD rats were randomly selected and divided into 3 groups,the control group,the model group,and the MSC-derived exosme treatment group(Exosome group)with 10 rats in each group.The survival rate,the level of renal function,the level of blood inflammatory mediators and proliferating cell nuclear antigen(PCNA)in renal tissue were compared among the three groups.Results Compared with the Model group,the renal blood creatinine and urea nitrogen levels of the Exosome group were significantly reduced,and the difference was statistically significant(P<0.05).After 6 days of exosome treatment,serum IL-6,TNF-αand IL-1βdecreased significantly(P<0.05).PCNA proliferation test showed that the number of proliferating epithelial cells in model group increased,and the number of proliferating cells in the Exosome group was more obvious.Conclusion The exosomes derived from umbilical cord mesenchymal stem cells have protective effect on acute kidney injury in rats with sepsis,and can improve renal function,reduce the level of inflammation and increase the level of renal tissue cell proliferation.

关 键 词：脐带间充质干细胞 外泌体 脓毒症 急性肾损伤 

分 类 号：R692[医药卫生—泌尿科学] R459.7[医药卫生—外科学]