不同滴灌模式对东北春播玉米籽粒淀粉积累及淀粉相关酶活性的影响  

Effects of Different Drip Irrigation Modes on Starch Accumulation and Activities of Starch Synthesis-Related Enzyme of Spring Maize Grain in Northeast China

作　　者：张家桦 杨恒山[1] 张玉芹[1] 李从锋[2] 张瑞富[1] 邰继承[1] 周阳晨 ZHANG JiaHua;YANG HengShan;ZHANG YuQin;LI CongFeng;ZHANG RuiFu;TAI JiCheng;ZHOU YangChen(College of Agronomy,Inner Mongolia Minzu University,Tongliao 028000,Inner Mongolia;Institute of Crop Sciences,Chinese Academy of Agricultural Sciences,Beijing 100081)

机构地区：[1]内蒙古民族大学农学院,内蒙古通辽028000 [2]中国农业科学院作物科学研究所,北京100081

出　　处：《中国农业科学》2022年第7期1332-1345,共14页Scientia Agricultura Sinica

基　　金：国家自然科学基金(31960382);内蒙古自然科学基金(2018LH03012,2018LH03007);国家重点研发计划(2017YFD0300805)。

摘　　要：【目的】探明不同滴灌模式对春玉米籽粒淀粉积累及淀粉合成相关酶活性的影响,为提高春玉米产量提供理论指导。【方法】以农华 101 为试验材料,采用裂区试验,主处理为滴灌模式,设膜下滴灌和浅埋滴灌 2 种,副处理为灌水量,设传统灌水量 40%(W1:1 440 m^(3)·hm^(-2))、传统灌水量 50%(W2:1 800 m^(3)·hm^(-2))和传统灌水量 60%(W3:2 160 m^(3)·hm^(-2))3 个水平,于苗期-拔节期、拔节期-大喇叭口期、大喇叭口期-吐丝期、吐丝期-乳熟期、乳熟期-收获按照 1﹕2﹕2﹕3﹕2比例滴灌。吐丝后 20 d 开始,每 7 d 取玉米籽粒 1 次,采用酸水解-DNS 法测定玉米籽粒中总淀粉含量,采用淀粉合成酶试剂盒测定腺苷二磷酸葡萄糖焦磷酸化酶(AGPase)、结合态淀粉合成酶(GBSS)、可溶性淀粉合成酶(SSS)活性,研究玉米籽粒产量、淀粉积累量特征及 AGPase、GBSS、SSS 酶活性变化。【结果】2019-2020 年春玉米有效穗数和穗粒数两种模式间差异不显著, W1 处理浅埋滴灌千粒重和产量低于膜下滴灌,W2 处理二者无显著性差异,W3 处理显著高于膜下滴灌,其中产量分别高 5.7%和 8.4%,千粒重分别高 11.9%、12.1%。方差分析表明,灌水量、滴灌模式和灌水量与滴灌模式互作对千粒重影响差异达显著、极显著水平,年份、灌水量及灌水量与滴灌模式互作对籽粒产量影响达到显著或极显著水平。W1 处理浅埋滴灌籽粒淀粉含量和积累量低于膜下滴灌,W2 处理从吐丝后 20 d 起浅埋滴灌低于膜下滴灌,吐丝后 55 d 二者差异不显著,W3 处理吐丝 41 d 前低于膜下滴灌,随生育进程差异减小,吐丝后 55 d 显著高于膜下滴灌。3 个灌水量下浅埋滴灌的籽粒淀粉活跃积累期较膜下滴灌时间长,且籽粒淀粉积累达到最大速率的时间较膜下滴灌时间延后。活跃积累期对淀粉最终积累量影响系数最大,其次为达到最大速率的时间,生育后期淀粉积累速率【Objective】The aim of this study was to explore the effects of different drip irrigation modes on starch accumulation and starch synthesis-related enzyme activities of spring maize, so as to provide a physiological basis for the understanding of the yield formation process in maize under the water-saving conditions.【Method】 Using maize variety Nonghua 101 as the experimental material, a sub-plot design was selected for this experiment, and two irrigation modes were chosen as the main plot, including mulched drip irrigation (MDI) and shallow drip irrigation (SBDI), and the irrigation amount as the sub-plot included three irrigation levels (W1: 1 440 m^(3)·hm^(-2);W2: 1 800 m^(3)·hm^(-2);W3: 2 160 m^(3)·hm^(-2), which equaled to the amount of 40%, 50% and 60% of the traditional irrigation, respectively). The amount and time of drip irrigation was conducted at the ratio of 1﹕2﹕2﹕3﹕2 for seedling stage to jointing stage, jointing stage to big bell mouth, big bell mouth to silking stage, silking stage to milk stage, and milk stage to harvest stage, respectively. The corn kernels were taken at every 7 days from 20 days after silking. The content of total starch in maize kernel was determined by acid hydrolysis DNS method. The starch synthase activity assay kit was used to determine the activity of adenosine diphosphate glucose pyro phosphorylase (ADPase), bound starch synthase (GBSS), and soluble starch synthase (SSS). On basis of that, the maize grain yield, characteristics of starch accumulation, and the changes enzyme activity of ADPase, GBSS and SSS were investigaged.【Result】There was no significant difference between effective panicle number and grain number per panicle of the two irrigation modes, and the 1000-grain weight and grain yield in shallow drip irrigation was lower than that of mulched drip irrigation under the treatment of W1, but no significant difference were found under the treatment of W2. The grain yield and 1000-grain weight in shallow drip irrigation were 5.7% and 8.4% hig

关 键 词：滴灌模式 春玉米 籽粒 酶活性 淀粉积累 

分 类 号：S513[农业科学—作物学]