丹参多酚酸盐对心肌细胞氧化损伤模型大鼠的作用  

EFFECT OF SALVIANOLATE ON MODEL RATS WITH OXIDATIVE DAMAGE OF CARDIOMYOCYTES

作　　者：高洪瑞 闫慧 潘洋 郝岩 李健[2] GAO Hongrui;YAN Hui;PAN Yang;HAO Yan;LI Jian(Medical Colleage of Qingdao University,Qingdao 266071,China)

机构地区：[1]青岛大学医学部,山东青岛266071 [2]青岛大学附属医院心内科,山东青岛266071

出　　处：《青岛大学学报（医学版）》2022年第2期189-192,共4页Journal of Qingdao University(Medical Sciences)

基　　金：山东省自然科学基金面上项目(ZR2017MH056)。

摘　　要：目的 探讨丹参多酚酸盐对黄曲霉素B1(AFB1)诱导下大鼠心肌细胞(H9C2细胞)氧化应激损伤的影响。方法 用不同浓度的AFB1处理H9C2细胞,通过CCK-8法检测细胞活力以确定最佳氧化损伤模型AFB1浓度(128μmol/L)。实验分为正常对照组(用DMEM培养液培养H9C2细胞)、AFB1诱导模型组(在正常对照组基础上加入128μmol/L AFB1)和低、中、高浓度丹参多酚酸盐干预组(128μmol/L AFB1+丹参多酚酸盐浓度分别为5、20、40 mg/L)。采用CCK-8法测定各组细胞的存活率,倒置相差显微镜观察细胞形态学变化,DCFH-DA染色检测细胞内活性氧(ROS)水平,Western blot方法检测氧化应激相关蛋白过氧化物歧化酶2(SOD2)和过氧化氢酶(CAT)蛋白的表达。结果 与正常对照组比较,AFB1诱导模型组的细胞形态发生明显改变,细胞相对存活率降低,细胞内ROS升高,细胞内SOD2、CAT蛋白表达降低;与AFB1诱导模型组相比较,高浓度丹参多酚酸盐干预组的细胞形态有所改善,细胞相对存活率增加,细胞内ROS明显降低,SOD2、CAT蛋白表达有所上调,差异均有统计学意义(F=8.023~226.937,P<0.05)。结论 丹参多酚酸盐可通过改善抗氧化酶活力、减少ROS的产生、减少氧化应激损伤,改善AFB1诱导的H9C2心肌细胞损伤。Objective To study the effect of salvianolate on oxidative damage of rat cardiomyocytes(H9C2) induced by aflatoxin B1(AFB1). Methods H9C2 cells were treated with different concentrations of AFB1, and the optimal concentration of AFB1(128 μmol/L) for the oxidative damage model was determined by CCK-8 assay. H9C2 cells were divided into normal group(cultured in DMEM medium), AFB1-induced model group(cultured in DMEM medium with 128 μmol/L AFB1), and intervention groups with low, medium, and high concentrations of salvianolate(128 μmol/L AFB1 + 5, 20, and 40 mg/L salvianolate). Cell survival rate was determined by CCK-8 assay. Morphological changes of cells were observed under an inverted phase contrast microscope. Intracellular reactive oxygen species(ROS) was detected by DCFH-DA staining. Western blot was used to measure the expression levels of stress-related superoxide dismutase 2(SOD2) and catalase(CAT). Results Compared with the normal group, the AFB1-induced model group showed significantly changed cell morphology, reduced cell survival rate, increased ROS production, and decreased protein expression of SOD2 and CAT. Compared with the AFB1-induced model group, the high-concentration salvianolate intervention group showed improved cell morphology, increased cell survival rate, significantly decreased ROS production, and up-regulated SOD2 and CAT(F=8.023-226.937,P<0.05). Conclusion Salvianolate can ameliorate AFB1-induced H9C2 damage by increasing the activity of antioxidant enzymes and reducing ROS production and related oxidative stress.

关 键 词：丹参多酚酸盐 黄曲霉素B1 肌细胞 心脏 氧化性应激 活性氧 

分 类 号：R542.2[医药卫生—心血管疾病] R282.71[医药卫生—内科学]