创伤性膝骨关节炎模型兔软骨下骨的影像学结构改变  

Imaging structural changes of subchondral bone in model rabbits with traumatic knee osteoarthritis

作　　者：张文凯 党晓谦[1] ZHANG Wenkai;DANG Xiaoqian(The First Department of Orthopedics,the Second Affiliated Hospital of Xi′an Jiaotong University,Xi′an,Shanxi 710004,China;The Third Department of Orthopedics,Xianyang Central Hospital,Xianyang,Shanxi 712099,China)

机构地区：[1]西安交通大学第二附属医院骨一科,710004 [2]陕西省咸阳市中心医院骨三科,712099

出　　处：《重庆医学》2022年第8期1285-1289,1296,共6页Chongqing medicine

摘　　要：目的基于动物模型观察创伤性膝骨关节炎(OA)和闭合损伤性膝OA中股骨远端和胫骨近端软骨下骨的影像学结构改变。方法将36只巨型花明兔随机分为空白组、创伤组、闭合损伤组,每组12只。除空白组外其余各组分别建立创伤性膝OA(创伤组)和闭合损伤性膝OA(闭合损伤组)动物模型,12周后各组动物行膝关节X线片及MRI检查,并在Micro CT下对其股骨远端、胫骨近端行骨形态结构三维重建和软骨下骨显微结构的测定。结果X线片及MRI检查结果显示:与空白组比较,创伤组X线片示膝关节间隙变窄,股骨远端和胫骨近端均有明显骨赘形成,MRI示膝关节滑膜增生、韧带及半月板退变、软骨及软骨下骨损伤,呈明显OA改变;闭合损伤组X线片示膝关节间隙尚可,股骨远端和胫骨近端有少量的骨赘形成,MRI示膝关节滑膜轻度增生,外侧半月板轻度肥厚,软骨下骨轻度增生,呈轻度OA改变。Micro CT检查:创伤组软骨下骨有骨小梁增宽、致密、排列紊乱等表现,关节内可见骨赘形成,骨体积分数(BVF)、骨小梁厚度(Tb·h)、骨小梁数量(Tb·n)较空白组均明显降低,骨小梁间隙(Tb·Sp)、体积骨密度(vBMD)和组织骨密度(tBMD)较空白组均明显增高;而闭合损伤组骨形态结构和骨显微结构参数较创伤组改变较轻;2组分别与空白组比较差异均有统计学意义(P<0.05)。结论软骨下骨结构的改变随着OA的形成和发展愈加明显,呈恶性循环,且创伤后形成OA的概率较闭合性损伤更大、更严重,因此,软骨下骨的结构改变对于OA的治疗有着重要意义。Objective To observe the structural changes of the imaging of the distal femur and proximal tibial subchondral bone in traumatic knee osteoarthritis(OA)and closed-injury knee OA based on animal models.Methods A total of 36 giant flowering rabbits were randomly divided into the blank group,the trauma group and the closed-injury group,with 12 cases in each group.Animal models of traumatic knee OA(the trauma group)and closed injury knee OA(the closed injury group)were established in each group except the blank group.X-ray and MRI examinations of knee joints were observed after 12 weeks.Three-dimensional reconstruction of bone morphological structure was performed and microscopic structure of subchondral bone was tested on the distal femur and proximal humerus under Micro CT.Results X-ray of the trauma group showed narrowing of the knee joint space,and the osteophyte formation was observed in the distal femur and proximal tibia,when compared with the blank group.MRI of the trauma group showed synovial hyperplasia of the knee joint,ligament and meniscus degeneration,cartilage and subchondral bone injury,and obvious OA change.X-ray of the closed injury group showed that the knee joint space was acceptable,and a small amount of osteophyte was formed at the distal femur and proximal tibia.MRI of the trauma group showed mild hyperplasia of the knee joint synovium,light degree of hypertrophy the lateral meniscus,mild hyperplasia of the subchondral bone,and light OA change.Micro CT examination of the trauma group showed the subchondral bone has widened,dense and disordered trabecular bone,and the formation of osteophyte was visible in the joint.The bone volume fraction(BVF),trabecular thickness(Tb·h)and trabecular bone number(Tb·n)were significantly reduced,and the trabecular bone space(Tb·Sp),volumetric bone density(vBMD)and the bone mineral density(tBMD)were significantly increased,when compared with the blank group.The bone morphology and bone microstructural coefficient of the closed injury group were lighter than th

关 键 词：骨关节炎 股骨远端 胫骨近端 软骨下骨 Micro CT 

分 类 号：R684.3[医药卫生—骨科学]