Simultaneous Quantification of Five Stereoisomeric Hexoses in Nine Biological Matrices Using Ultrahigh Performance Liquid Chromatography with Tandem Mass Spectrometry  

作　　者：Jiali Zuo Runxian Cai Yanpeng An Huiru Tang 

机构地区：[1]State Key Laboratory of Genetic Engineering,Metabonomics and Systems Biology Laboratory At Shanghai International Centre for Molecular Phenomics,Zhongshan Hospital and School of Life Sciences,Human Phenome Institute,Fudan University,Shanghai 200438,China

出　　处：《Journal of Analysis and Testing》2020年第4期249-256,共8页分析检测（英文）

基　　金：support from the National Key R&D Program of China(2017YFC0906800);Shanghai Municipal Science and Technology Major Project(2017SHZDZX01);the National Natural Science Foundation of China(81590953,31821002 and 21405020).

摘　　要：Stereoisomeric hexoses are present in almost all biological organisms in the forms of aldoses and ketoses,with diverse physi-ological and pathophysiological functions.Accurate and simultaneous quantification is vital for understanding their functions individually.However,such analysis remains challenging owing to their highly similar behavior in chromatography and mass spectrometry.By combining the pre-column 3-nitrophenylhydrazine derivatization and ultrahigh performance liquid chroma-tography tandem mass spectrometry(UHPLC-MS/MS),here,we developed a method for simultaneous quantification of five important stereoisomeric hexoses including D-glucose,D-galactose,D-mannose,D-fructose and L-sorbose representing both aldoses and ketoses.The method achieved baseline-separation for all these five derivatized hexoses chromatographically and had high sensitivity(LOD,femtomole on column),excellent linearity(R2>0.995)and efficiency with stable-isotope dilution.With this method,we further quantified these hexoses in nine biological matrices including human biofluids(serum,urine and saliva),human cells,human and mouse feces,rat liver tissue,mung-bean seeds and peach pulp.The results provided quantitative data for these hexoses in multiple biological samples and showed significant concentration diversity for these hexoses in different biological samples,which demonstrated the applicability of the method for simultaneous quantification of these hexose phenotypes of biological systems.

关 键 词：HEXOSE Simultaneous quantification Biological matrices 3-nitrophenylhydrazine Ultrahigh performance liquid chromatography-tandem mass spectrometry 

分 类 号：O657.63[理学—分析化学]